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Photophysics and photochemistry of zinc, aluminium and tin octakis (benzylthio) phthalocyanines
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.ORCID-id: 0000-0003-0578-4003
2008 (engelsk)Rapport (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
2008.
Serie
Trita-FYS, ISSN 0280-316X ; 2008:44
Emneord [en]
Zinc, aluminum and tin benzylthio phthalocyanines, singlet oxygen, triplet yield, triplet lifetime
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-10051OAI: oai:DiVA.org:kth-10051DiVA, id: diva2:202368
Merknad
QC 20101102Tilgjengelig fra: 2009-03-10 Laget: 2009-03-10 Sist oppdatert: 2010-11-02bibliografisk kontrollert
Inngår i avhandling
1. Photophysical and Chemical Approaches to Cellular Biophysics
Åpne denne publikasjonen i ny fane eller vindu >>Photophysical and Chemical Approaches to Cellular Biophysics
2008 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

The central theme in this thesis is reversibility. Two main attempts has been made to approach reversibility in cellular systems from both chemical and physical points of view. Reversibility of immunolabeling of proteins on the cell surface has been adressed by development of new fluorescent substances optimized for CALI (Chromophore-Assisted Laser Inactivation of protein). Aluminum phthalocyanine (AlPc) is here identified to be a good candidate for a new generation of fluorophores for efficient hydroxyl radical generation. It is shown that cells can be reversibly labeled with antibody-AlPc conjugates. In experiments on living cells the AlPcs were not only active as classic fluorophores but also as photocatalytic substances with destaining properties. Reversibility of cell immobilization is also reported, where cells cultured in microstructures were immobilized and 3D supported using hydrogels. Hydrogel formulation and application was optimized to achieve a system where both viability and ease of use was satisfied. Gel reversibility was actualized with pH and enzyme treatment. The developped method offers the possibility of stop flow culturing cells in controlled and reusable 3D environments.

sted, utgiver, år, opplag, sider
Stockholm: KTH, 2008. s. v, 26
Serie
Trita-FYS, ISSN 0280-316X ; 2008:54
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-10097 (URN)978-91-7412-200-5 (ISBN)
Presentation
2008-12-18, FA 32, AlbaNova Universitetscentrum, Roslagstullsbacken 21, Stockholm, 11:00 (engelsk)
Opponent
Veileder
Merknad
QC 20101102Tilgjengelig fra: 2009-03-16 Laget: 2009-03-16 Sist oppdatert: 2010-11-02bibliografisk kontrollert

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