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Sequence analysis of domestic dog mitochondrial DNA for forensic use
KTH, Tidigare Institutioner                               , Biokemi och biokemisk teknologi.ORCID-id: 0000-0002-1495-8338
KTH, Tidigare Institutioner                               , Biokemi och biokemisk teknologi.ORCID-id: 0000-0003-2745-6289
Vise andre og tillknytning
1997 (engelsk)Inngår i: Journal of Forensic Sciences, ISSN 0022-1198, E-ISSN 1556-4029, Vol. 42, nr 4, s. 593-600Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A method has been developed for the direct sequencing of hypervariable region 1 (HV1) of domestic dog (Canis familiaris) and wolf (Canis lupus) mitochondrial DNA (mtDNA) using single hairs as template. The method uses a robotic work-station and an automated sequencer to allow for robust routine analysis. A population data base was created in order to investigate the forensic and population-genetic informativeness of domestic dog HV1. Sequence variation, partitioning of dog breeds among sequence variants and phylogenetic relations between the variants were determined. Samples from 102 domestic dogs of 52 different breeds and two captive wolves were analyzed. Nineteen dog sequence variants were found and the frequencies of the variants ranged from 1 to 21%. The calculated discrimination power of the region, i.e., the exclusion capacity, implied that nine out of ten disputed individuals can be excluded by this analysis. The sequence variants were found to cluster into four phylogenetic groups.

sted, utgiver, år, opplag, sider
1997. Vol. 42, nr 4, s. 593-600
Emneord [en]
forensic science, DNA typing, dog, Canis familiaris, mitochondrial DNA, sequence analysis, hair, SINGLE HAIRS, IDENTIFICATION, VARIABILITY, POPULATION, FAMILY, PCR
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-13928ISI: A1997XL34800004OAI: oai:DiVA.org:kth-13928DiVA, id: diva2:328263
Merknad
QC 20100702Tilgjengelig fra: 2010-07-02 Laget: 2010-07-02 Sist oppdatert: 2020-01-10bibliografisk kontrollert
Inngår i avhandling
1. Mitochondrial DNA: analysis of the control region in forensic and population genetic studies
Åpne denne publikasjonen i ny fane eller vindu >>Mitochondrial DNA: analysis of the control region in forensic and population genetic studies
1999 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Stockholm: KTH, 1999. s. 49
Emneord
mitochondrial DNA, control region, tandem repeats, D-loop, homoplasmy, hair, forensic, microsatellites, evolution, DNA sequencing, PCR, domestic dog, wolf
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-2824 (URN)91-7170-408-6 (ISBN)
Disputas
1999-06-11, 00:00
Merknad
QC 20100702Tilgjengelig fra: 2000-01-01 Laget: 2000-01-01 Sist oppdatert: 2010-07-02bibliografisk kontrollert

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