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Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems
2005 (Engelska)Ingår i: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 7, nr 4, s. 607-613Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Recently (Dvinskikh et al., J. Magn. Reson., 2003, 164, 165 and Dvinskikh et al., J. Magn. Reson., 2004, 168, 194), some of us introduced two efficient solid-state NMR techniques for the determination of heteronuclear dipolar couplings under magic-angle spinning (MAS). These two-dimensional (2D) recoupling methods have been applied previously to simple amino acids, and to columnar systems with high positional and orientational order. In this work, we show that the 2D MAS sequences produce unparalleled H-1-C-13 dipolar resolution in unoriented lipid membranes. The recoupling experiments were applied to hydrated dimyristoylphosphatidylcholine (DMPC) in the liquid-crystalline L-alpha phase, and the results agreed well with previous NMR investigations using specifically deuterated phospholipids.

Ort, förlag, år, upplaga, sidor
2005. Vol. 7, nr 4, s. 607-613
Nyckelord [en]
nuclear-magnetic-resonance, angle-spinning nmr, goldburg cross-polarization, magic-angle, mas nmr, pulse sequences, liquid-crystals, c-13, spectroscopy, bilayers
Identifikatorer
URN: urn:nbn:se:kth:diva-14575DOI: 10.1039/b418131jISI: 000227386600007PubMedID: 19787876Scopus ID: 2-s2.0-14644399800OAI: oai:DiVA.org:kth-14575DiVA, id: diva2:332616
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QC 20100525Tillgänglig från: 2010-08-05 Skapad: 2010-08-05 Senast uppdaterad: 2020-05-11Bibliografiskt granskad

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