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Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes
KTH, Skolan för bioteknologi (BIO).
KTH, Skolan för bioteknologi (BIO).
KTH, Skolan för bioteknologi (BIO).
Vise andre og tillknytning
2005 (engelsk)Inngår i: Bioprocess and biosystems engineering (Print), ISSN 1615-7591, E-ISSN 1615-7605, Vol. 27, nr 6, s. 399-406Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

An oxygen-limited fed-batch technique (OLFB) was compared to traditional methanol-limited fed-batch technique (MLFB) for the production of recombinant Thai Rosewood beta-glucosidase with Pichia pastoris. The degree of energy limitation, expressed as the relative rate of respiration (q(O)/q(O,max)), was kept similar in both the types of processes. Due to the higher driving force for oxygen transfer in the OLFB, the oxygen and methanol consumption rates were about 40% higher in the OLFB. The obligate aerobe P. pastoris responded to the severe oxygen limitation mainly by increased maintenance demand, measured as increased carbon dioxide production per methanol, but still somewhat higher cell density (5%) and higher product concentrations (16%) were obtained. The viability was similar, about 90-95%, in both process types, but the amount of total proteins released in the medium was much less in the OLFB processes resulting in substantially higher (64%) specific enzyme purity for input to the downstream processing.

sted, utgiver, år, opplag, sider
2005. Vol. 27, nr 6, s. 399-406
Emneord [en]
oxygen-limited fed batch (OLFB), methanol-limited fed batch (MLFB), Pichia pastoris, b-glucosidase, methanol, expression, yeast, growth, yield, oxidation
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-15170DOI: 10.1007/s00449-005-0005-4ISI: 000233136000006Scopus ID: 2-s2.0-28244476895OAI: oai:DiVA.org:kth-15170DiVA, id: diva2:333211
Merknad

QC 20100525

Tilgjengelig fra: 2010-08-05 Laget: 2010-08-05 Sist oppdatert: 2017-12-12bibliografisk kontrollert
Inngår i avhandling
1. Process design for production of Thai rosewood β-glucosidase in Pichia pastoris
Åpne denne publikasjonen i ny fane eller vindu >>Process design for production of Thai rosewood β-glucosidase in Pichia pastoris
2005 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Stockholm: KTH, 2005. s. 50
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-375 (URN)91-7178-081-5 (ISBN)
Presentation
2005-06-10, FB54, AlbaNova, Roslagstullsbacken 21, Stockholm, 11:00
Veileder
Merknad
QC 20101126Tilgjengelig fra: 2005-08-08 Laget: 2005-08-08 Sist oppdatert: 2010-11-26bibliografisk kontrollert

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Charoenrat, TheppanyaStendahl-Andersen, HelleJahic, MehmedalijaEnfors, Sven-Olof
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