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High-resolution computed tomography with a compact soft x-ray microscope
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Experimentell biomolekylär fysik.ORCID-id: 0000-0002-4394-0591
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.ORCID-id: 0000-0003-2745-6289
Visa övriga samt affilieringar
2009 (Engelska)Ingår i: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 17, nr 13, s. 11057-11065Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Computed tomography based on high-resolution soft x-ray microscopy utilizes the natural contrast for biological specimens provided by the water window (lambda = 2.4 - 4.4 nm) and the high resolving power of zone plate objectives. It is capable of revealing the 3D structure of biological specimens at sub-visible-microscopic resolution. To date, the technique has only been available at synchrotron-based microscopes, which limits the researchers access. In the present paper we demonstrate high-resolution soft x-ray tomography with a laboratory zone-plate-based soft x-ray microscope. The specimen, a diatom mounted on a glass capillary, was reconstructed from a tilt series of 53 images covering 180 using a filtered back projection algorithm. The resolution of the tomogram was estimated to a half period of 140 nm using a differential-phase-residual method. Cryo-fixation, increased source brightness and extended-depth-of-focus objectives are important for pushing the resolution of compact systems for biological samples.

Ort, förlag, år, upplaga, sidor
2009. Vol. 17, nr 13, s. 11057-11065
Identifikatorer
URN: urn:nbn:se:kth:diva-18584ISI: 000267761100066Scopus ID: 2-s2.0-67649271251OAI: oai:DiVA.org:kth-18584DiVA, id: diva2:336631
Anmärkning
QC 20100525Tillgänglig från: 2010-08-05 Skapad: 2010-08-05 Senast uppdaterad: 2020-01-30Bibliografiskt granskad
Ingår i avhandling
1. Phase-Contrast and High-Resolution Optics for X-Ray Microscopy
Öppna denna publikation i ny flik eller fönster >>Phase-Contrast and High-Resolution Optics for X-Ray Microscopy
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

X-ray microscopy is a well-established technique for nanoscale imaging. Zone plates are used as microscope objectives and provide high resolution, approaching 10 nm, currently limited by fabrication issues. This Thesis presents zone plate optics that achieve either high resolution or phase contrast in x-ray microscopy. The high-resolution optics use high orders of the zone plate, which alleviates the demands on fabrication, and the phase-contrast optics are single-element diffractive optical elements that produce contrast by Zernike or differential-interference contrast methods. The advantage of phase contrast in x-ray microscopy is shorter exposure times, and is crucial in the hard x-ray regime. Microscopy in the absorption‑contrast region of the water-window (2.34 - 4.37 nm) also benefits from these optics. The development of the optics for a laboratory soft x-ray microscope spans from theoretical and numerical analysis of coherence and stray light to experimental implementation and testing. The laboratory microscope uses laser-produced plasma-sources in the water-window and is unique in its design and performance. It will be shown that the laboratory microscope in its current form is a user-oriented and stable instrument, and has been used in a number of applications. The implementation of a cryogenic sample stage for tomographic imaging of biological samples in their natural environment has enabled applications in biology, and 3D x-ray microscopy of cells was performed for the first time with a laboratory instrument.

 

Ort, förlag, år, upplaga, sidor
Stockholm: KTH, 2010. s. x, 61
Serie
Trita-FYS, ISSN 0280-316X ; 72
Nyckelord
Microscopy, X-ray optics, X-ray microscopy, Soft X-ray physics
Nationell ämneskategori
Atom- och molekylfysik och optik Fysik
Identifikatorer
urn:nbn:se:kth:diva-26781 (URN)978-91-7415-834-2 (ISBN)
Disputation
2010-12-17, FD5, Roslagstullsbacken 21, Stockholm, 15:42 (Engelska)
Opponent
Handledare
Anmärkning
QC 20101130Tillgänglig från: 2010-11-30 Skapad: 2010-11-26 Senast uppdaterad: 2010-11-30Bibliografiskt granskad
2. Laboratory soft x-ray microscopy and tomography
Öppna denna publikation i ny flik eller fönster >>Laboratory soft x-ray microscopy and tomography
2011 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Soft x-ray microscopy in the water-window (λ = 2.28 nm – 4.36 nm) is based on zone-plate optics and allows high-resolution imaging of, e.g., cells and soils in their natural or near-natural environment. Three-dimensional imaging is provided via tomographic techniques, soft x-ray cryo tomography. However, soft x-ray microscopes with such capabilities have been based on large-scale synchrotron x‑ray facilities, thereby limiting their accessibility for a wider scientific community.

This Thesis describes the development of the Stockholm laboratory soft x-ray microscope to three-dimensional cryo tomography and to new optics-based contrast mechanisms. The microscope relies on a methanol or nitrogen liquid-jet laser-plasma source, normal-incidence multilayer or zone-plate condenser optics, in-house fabricated zone-plate objectives, and allows operation at two wavelengths in the water-window, λ = 2.48 nm and λ = 2.48 nm. With the implementation of a new state-of-the-art normal-incidence multilayer condenser for operation at λ = 2.48 nm and a tiltable cryogenic sample stage the microscope now allows imaging of dry, wet or cryo-fixed samples. This arrangement was used for the first demonstration of laboratory soft x-ray cryo microscopy and tomography. The performance of the microscope has been demonstrated in a number of experiments described in this Thesis, including, tomographic imaging with a resolution of 140 nm, cryo microscopy and tomography of various cells and parasites, and for studies of aqueous soils and clays. The Thesis also describes the development and implementation of single-element differential-interference and Zernike phase-contrast zone-plate objectives. The enhanced contrast provided by these optics reduce exposure times or lowers the dose in samples and are of major importance for harder x-ray microscopy. The implementation of a high-resolution 50 nm compound zone-plate objective for sub-25-nm resolution imaging is also described. All experiments are supported by extensive numerical modelling for improved understanding of partially coherent image formation and stray light in soft x-ray microscopes. The models are useful tools for studying effects of zone plate optics or optical design of the microscope on image formation and quantitative accuracy in soft x-ray tomography.

Ort, förlag, år, upplaga, sidor
Stockholm: KTH Royal Institute of Technology, 2011. s. x, 76
Serie
Trita-FYS, ISSN 0280-316X ; 2011:03
Nyckelord
Microscopy, X-ray optics, Diffractive optics, Zone plates, X-ray microscopy, Soft X-ray physics, Tomography
Nationell ämneskategori
Atom- och molekylfysik och optik Fysik
Identifikatorer
urn:nbn:se:kth:diva-29950 (URN)978-91-7415-874-8 (ISBN)
Disputation
2011-02-25, FB42, AlbaNova Universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:00 (Engelska)
Opponent
Handledare
Anmärkning
QC 20110221Tillgänglig från: 2011-02-21 Skapad: 2011-02-18 Senast uppdaterad: 2011-02-21Bibliografiskt granskad

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