Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-5584-9170
KTH, School of Information and Communication Technology (ICT), Microelectronics and Applied Physics, MAP.ORCID iD: 0000-0002-2082-9583
2009 (English)In: Applied Optics, ISSN 1559-128X, E-ISSN 2155-3165, Vol. 48, no 31, p. 6050-6058Article in journal (Refereed) Published
Abstract [en]

The theory of autocorrelation-function evaluation in fluorescence correlation spectroscopy is applied to a Lorentzian intensity distribution. An analytical solution to the autocorrelation function for diffusion is deduced for this spatial distribution. Experimental investigation of the distribution is performed using an enlarged detector aperture in a standard confocal setup. The data from the experiment are fitted to the derived autocorrelation function, and a reasonable estimate of the spatial distribution is provided. Estimates are also compared to values computed by molecular detection efficiency simulation. The use of Lorentzian intensity distributions complements conditions where a Gaussian intensity distribution applies, expanding the applicability range of analytical correlation functions.

Place, publisher, year, edition, pages
2009. Vol. 48, no 31, p. 6050-6058
Keywords [en]
internal-reflection fluorescence, mode wave-guides, high count-rate, fluctuation spectroscopy, detection volumes, diffusion, dynamics, probe, microscopy, artifacts
Identifiers
URN: urn:nbn:se:kth:diva-18927DOI: 10.1364/AO.48.006050ISI: 000271374000047PubMedID: 19881673Scopus ID: 2-s2.0-70449623657OAI: oai:DiVA.org:kth-18927DiVA, id: diva2:336974
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2020-03-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Blom, HansBjörk, Gunnar

Search in DiVA

By author/editor
Blom, HansBjörk, Gunnar
By organisation
Applied PhysicsMicroelectronics and Applied Physics, MAP
In the same journal
Applied Optics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 334 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf