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Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0002-5584-9170
KTH, Skolan för informations- och kommunikationsteknik (ICT), Mikroelektronik och tillämpad fysik, MAP.ORCID-id: 0000-0002-2082-9583
2009 (engelsk)Inngår i: Applied Optics, ISSN 1559-128X, E-ISSN 2155-3165, Vol. 48, nr 31, s. 6050-6058Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The theory of autocorrelation-function evaluation in fluorescence correlation spectroscopy is applied to a Lorentzian intensity distribution. An analytical solution to the autocorrelation function for diffusion is deduced for this spatial distribution. Experimental investigation of the distribution is performed using an enlarged detector aperture in a standard confocal setup. The data from the experiment are fitted to the derived autocorrelation function, and a reasonable estimate of the spatial distribution is provided. Estimates are also compared to values computed by molecular detection efficiency simulation. The use of Lorentzian intensity distributions complements conditions where a Gaussian intensity distribution applies, expanding the applicability range of analytical correlation functions.

sted, utgiver, år, opplag, sider
2009. Vol. 48, nr 31, s. 6050-6058
Emneord [en]
internal-reflection fluorescence, mode wave-guides, high count-rate, fluctuation spectroscopy, detection volumes, diffusion, dynamics, probe, microscopy, artifacts
Identifikatorer
URN: urn:nbn:se:kth:diva-18927ISI: 000271374000047Scopus ID: 2-s2.0-70449623657OAI: oai:DiVA.org:kth-18927DiVA, id: diva2:336974
Merknad
QC 20100525Tilgjengelig fra: 2010-08-05 Laget: 2010-08-05 Sist oppdatert: 2017-12-12bibliografisk kontrollert

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Blom, HansBjörk, Gunnar

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