Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Mammalian cell production of a respiratory syncytial virus (RSV) candidate vaccine recovered using a product-specific affinity column
KTH, Tidigare Institutioner                               , Bioteknologi.ORCID-id: 0000-0003-4214-6991
Vise andre og tillknytning
2001 (engelsk)Inngår i: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 34, s. 25-32Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The recombinant production of a respiratory syncytial virus (RSV) candidate vaccine BBG2Na in baby hamster kidney cells (BHK-21 cells) was investigated. BBG2Na consists of a serum-albumin-binding region (BB) fused to a 101-amino-acid fragment of the RSV G-protein. Semliki Forest virus-based expression vectors encoding both intracellular and secreted forms of BBG2Na were constructed and found to be functional. Affinity recovery of BBG2Na employing human serum albumin columns was found to be inefficient due to the abundance of BSA in the applied samples. Instead, a strategy using a tailor-made affinity ligand based on a combinatorially engineered Staphylococcus aureus protein A domain, showing specific binding to the G-protein part of the product, was evaluated. In conclusion, a strategy for production and successful recovery of BBG2Na in mammalian cells was created, through the development of a product-specific affinity column.

sted, utgiver, år, opplag, sider
2001. Vol. 34, s. 25-32
Emneord [en]
affibody, affinity chromatography, mammalian cell expression, Semliki Forest virus, Staphylococcus aureus protein A, bacterial receptor domain, taq dna-polymerase, g-protein fragment, combinatorial libraries, protective immunity, expression systems, fusion protein, binding, replicon, vectors
Identifikatorer
URN: urn:nbn:se:kth:diva-20911ISI: 000170684000005OAI: oai:DiVA.org:kth-20911DiVA, id: diva2:339608
Merknad
QC 20100525Tilgjengelig fra: 2010-08-10 Laget: 2010-08-10 Sist oppdatert: 2017-12-12bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Personposter BETA

Nygren, Per-ÅkeStåhl, Stefan

Søk i DiVA

Av forfatter/redaktør
Nygren, Per-ÅkeStåhl, Stefan
Av organisasjonen
I samme tidsskrift
Biotechnology and applied biochemistry

Søk utenfor DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric

urn-nbn
Totalt: 288 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf