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Measurement of interactions between protein layers adsorbed on silica by atomic force microscopy
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2004 (Engelska)Ingår i: Journal of Physics: Condensed Matter, ISSN 0953-8984, E-ISSN 1361-648X, Vol. 16, nr 26, s. S2383-S2392Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The present work, using an atomic force microscope and the colloid probe technique, investigates the interaction forces between bovine serum albumin (BSA) layers and between apoferritin layers adsorbed on silica surfaces. The measurements have been carried out in an aqueous medium at different pH values and NaCl concentrations. Similar behaviours have been found with both proteins. Electrostatic and steric forces dominate the interactions between the protein layers at low NaCl concentrations. However, a very strange behaviour is found as a function of pH at high NaCl concentrations. The results obtained under these conditions could be explained if the presence of hydration forces in these systems is assumed.

Ort, förlag, år, upplaga, sidor
2004. Vol. 16, nr 26, s. S2383-S2392
Nyckelord [en]
bovine serum-albumin, colloidal stability, hydration forces, apoferritin, scattering, adsorption, lysozyme, aggregation, membranes, cataract
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Kemi
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URN: urn:nbn:se:kth:diva-23625DOI: 10.1088/0953-8984/16/26/011ISI: 000223086300013Scopus ID: 2-s2.0-3142721401OAI: oai:DiVA.org:kth-23625DiVA, id: diva2:342324
Anmärkning
QC 20100525 QC 20110926. Meeting on Biological Surfaces and Interfaces. Castelvecchio Pascoli, ITALY. JUN, 2003 Tillgänglig från: 2010-08-10 Skapad: 2010-08-10 Senast uppdaterad: 2017-12-12Bibliografiskt granskad

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Feiler, AdamRutland, Mark W
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