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Signal Enhancement in Ligand-Receptor Interactions using Dynamic Polymers at Quartz Crystal Microbalance Surfaces
KTH, Skolan för kemivetenskap (CHE), Kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi.ORCID-id: 0000-0002-1533-6514
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-25122OAI: oai:DiVA.org:kth-25122DiVA, id: diva2:355897
Merknad
QC 20101008Tilgjengelig fra: 2010-10-08 Laget: 2010-10-08 Sist oppdatert: 2019-12-20bibliografisk kontrollert
Inngår i avhandling
1. Signal Enhancement by Dynamic Polymers in Quartz Crystal Microbalance Applications
Åpne denne publikasjonen i ny fane eller vindu >>Signal Enhancement by Dynamic Polymers in Quartz Crystal Microbalance Applications
2009 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

 This licentiate thesis presents studies of signal enhancement by dynamic polymers in quartz crystal microbalance (QCM) applications. The aim of the study has been to determine the potential for the use of polymers on QCM sensors for signal enhancement in molecular interaction studies. A method for synthesis of polymer sensor surfaces on QCM substrates was developed based on an iniferter photo polymerization technique. Polymerized poly(acrylic acid) and poly(acryl amide) surfaces were extensively characterized with electron spectroscopy for chemical analysis (ESCA), infrared reflection absorption spectroscopy (IRAS) and atomic force microscopy (AFM). The QCM response dynamics of these surfaces to pH changes was studied and the carboxyl containing surfaces exhibited large, reversible and highly reproducible frequency shifts due to expansion of deprotonated polymer chains. Surface acid dissociation constants (pKa) were determined for poly(acrylic acid) surfaces, carboxymethyldextran surfaces and self-assembled monolayer carboxyl surfaces by means of pH titration in QCM flow-through instrumentation. Surface pKa data was consistent with available literature data. The sensitivity enhancement for molecular interaction studies was investigated for poly(acrylic acid) coated QCM sensors with a model system consisting of immobilized biotin and an anti-biotin Fab fragment. Binding responses of antibiotin Fab to immobilized biotin was revealed to be 11 times higher than the corresponding results for a carboxylated SAM surface.

 

sted, utgiver, år, opplag, sider
Stockholm: KTH, 2009. s. 41
Serie
Trita-CHE-Report, ISSN 1654-1081 ; 2009:17
Emneord
Signal enhancement, QCM, polyelectrolytes, hydrogel, iniferter, photopolymerization, dynamic polymers
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-10305 (URN)978-91-7415-307-1 (ISBN)
Presentation
2009-05-27, E3, Osquars backe 14, Stockholm, 10:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2009-05-07 Laget: 2009-05-06 Sist oppdatert: 2010-10-15bibliografisk kontrollert

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