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Substrate Conformations Set the Rate of Enzymatic Acrylation by Lipases
KTH, Skolan för bioteknologi (BIO), Biokemi.ORCID-id: 0000-0002-4066-2776
KTH, Skolan för bioteknologi (BIO), Biokemi.
2010 (engelsk)Inngår i: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 11, nr 6, s. 802-810Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Acrylates represent a class of 4-unsaturated compounds of high industrial importance. We investigated the influence of substrate conformations on the experimentally determined reaction rates of the enzyme-catalysed transacylation of methyl acrylate and derivatives by ab initio DFT B3LYP calculations and molecular dynamics simulations. The results supported a least-motion mechanism upon the sp(2) to sp(3) substrate transition to reach the transition state in the enzyme active site. This was in accordance with our hypothesis that acrylates form productive transition states from their low-energy s-sis/s-trans conformations. Apparent k(cat) values were measured for Candida antarctica lipase B (CALB), Humicola insolens cutinase and Rhizomucor miehei lipase and were compared to results from computer simulations. More potent enzymes for acryltransfer, such as the CALB mutant V190A and acrylates with higher turnover numbers, showed elevated populations of productive transition states.

sted, utgiver, år, opplag, sider
2010. Vol. 11, nr 6, s. 802-810
Emneord [en]
acrylate, biocatalysis, enzymes, molecular modeling, protein design
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-27913DOI: 10.1002/cbic.200900758ISI: 000277299800013Scopus ID: 2-s2.0-77950664710OAI: oai:DiVA.org:kth-27913DiVA, id: diva2:383043
Merknad
QC 20110104Tilgjengelig fra: 2011-01-04 Laget: 2011-01-03 Sist oppdatert: 2020-05-11bibliografisk kontrollert
Inngår i avhandling
1. On electrostatic effects, minimal motion and other catalytic strategies used by enzymes
Åpne denne publikasjonen i ny fane eller vindu >>On electrostatic effects, minimal motion and other catalytic strategies used by enzymes
2011 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Enzymes are powerful biocatalysts that provide rate accelerations of up to 1019 fold compared to the corresponding uncatalyzed reaction in solution. The origin of the remarkable performance displayed by enzymes has fascinated and puzzled researchers for over a hundred years. It is clear that the catalytic effect is a consequence of the higher degree of transition state stabilization for the enzyme catalyzed reaction compared to the corresponding uncatalyzed reaction. It is still not well understood exactly how this transition state stabilization occurs and the relative importance of various catalytic effects are discussed. Catalytic effects involving electrostatics, near attack conformers, dynamic effects and an economy in atomic motion are discussed in this thesis.

The importance of electrostatic effects is corroborated in this thesis. A single hydrogen bond in transition state constitutes an important difference between amidases and esterases. A hydrogen bond in transition state is found in all sixteen analyzed amidases representing ten different reaction mechanisms and eleven different folding families. The hydrogen bond is shown to be either substrate assisted or enzyme assisted. The role of this hydrogen bond is to assist nitrogen inversion in amidases. Esterases lack this interaction in transition state and therefore they are very poor catalysts in the hydrolysis of amides. Electrostatic interactions are found to facilitate proton transfer that enhances the rate of lipase catalyzed N-acylation of amino alcohols.

In this thesis electrostatic effects in the substrate are shown to be important for the lipase catalyzed transacylation of acrylates The α,β-double bond present in acrylates introduce electronic effects that has the consequence of restricting the conformational freedom of the substrate in its ground state to two flat conformations, s-cis and s-trans. It is shown that acrylates form near attack conformers (NACs) from their ground state s-cis/s-trans planar conformations. The ability of the enzyme to accommodate such apparent s-cis/s-trans substrate conformations dictates the probability to form productive transition states and thus the reaction rate.

Dynamic effects are important in enzymes. In this thesis it is found that a point mutation increases the flexibility of a neighbouring residue in Candida antarctica lipase B. This allows the mutated enzyme to explore conformations not accessible for the wild-type enzyme. The dynamics has the effect to decrease steric interactions in transition state with concomitant rate increase for the transacylation of methyl methacrylate.

In this thesis an economy of atomic motion during enzyme catalysis is observed. Nitrogen inversion in amidases constitutes an interesting example. A rotation as part of the reaction mechanism for amide bond hydrolysis would involve much more motion.

sted, utgiver, år, opplag, sider
Stockholm: KTH Royal Institute of Technology, 2011. s. 42
Serie
Trita-BIO-Report, ISSN 1654-2312 ; 14
HSV kategori
Forskningsprogram
SRA - Molekylär biovetenskap
Identifikatorer
urn:nbn:se:kth:diva-33334 (URN)978-91-7415-976-9 (ISBN)
Disputas
2011-05-27, D2, Lindstedtsvägen 5, Stockholm, 13:00 (engelsk)
Opponent
Veileder
Merknad
QC 20110512Tilgjengelig fra: 2011-05-12 Laget: 2011-05-03 Sist oppdatert: 2011-05-12bibliografisk kontrollert

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