Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Suppression of Water as a Nucleophile in Candida antarctica Lipase B Catalysis
KTH, Skolan för bioteknologi (BIO), Biokemi.
KTH, Skolan för bioteknologi (BIO), Biokemi.
KTH, Skolan för bioteknologi (BIO), Biokemi.ORCID-id: 0000-0002-2993-9375
Vise andre og tillknytning
2010 (engelsk)Inngår i: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 11, nr 6, s. 796-801Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A water tunnel in Candida antarctica lipase B that provides the active site with substrate water is hypothesized. A small, focused library created in order to prevent water from entering the active site through the tunnel was screened for increased transacylation over hydrolysis activity. A single mutant, S47L, in which the inner part of the tunnel was blocked, catalysed the transacylation of vinyl butyrate to 20 mm butanol 14 times faster than hydrolysis. The single mutant Q46A, which has a more open outer end of the tunnel, showed an increased hydrolysis rate and a decreased hydrolysis to transacylation ratio compared to the wild-type lipase. Mutants with a blocked, tunnel could be very useful in applications in which hydrolysis is unwanted, such as the acylation of highly hydrophilic compounds in the presence of water.

sted, utgiver, år, opplag, sider
2010. Vol. 11, nr 6, s. 796-801
Emneord [en]
Candida antarctica lipase B, hydrolysis, library screening, mutagenesis, rational design, transacylation
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-27912DOI: 10.1002/cbic.200900743ISI: 000277299800012PubMedID: 20235107Scopus ID: 2-s2.0-77950635205OAI: oai:DiVA.org:kth-27912DiVA, id: diva2:383047
Merknad
QC 20110104Tilgjengelig fra: 2011-01-04 Laget: 2011-01-03 Sist oppdatert: 2020-05-11bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Martinelle, Mats

Søk i DiVA

Av forfatter/redaktør
Larsen, Marianne WittrupZielinska, Dorota F.Martinelle, MatsHult, Karl
Av organisasjonen
I samme tidsskrift
ChemBioChem (Print)

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 94 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf