The LCC fractionation process developed earlier by us for hardwood samples has been further improved to include an additional fractionation of glucomannan-lignin by a step of Ba(OH)(2) precipitation to suit softwood species better. Glucan-Lignin, Glucomannan-Lignin and Xylan-Lignin were thus obtained from spruce wood and subjected to SEC and NMR analysis and thioacidolysis followed by GC and SEC analysis respectively for structural elucidations. The LCC structures have been finally verified by comparisons with the LCC fractions prepared by another earlier developed softwood process.