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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0002-3627-6899
Vise andre og tillknytning
2011 (engelsk)Inngår i: PLOS ONE, ISSN 1932-6203, Vol. 6, nr 5, s. e19723-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

sted, utgiver, år, opplag, sider
2011. Vol. 6, nr 5, s. e19723-
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-34357DOI: 10.1371/journal.pone.0019723ISI: 000290771200019Scopus ID: 2-s2.0-79956258795OAI: oai:DiVA.org:kth-34357DiVA, id: diva2:432545
Forskningsfinansiär
FormasEU, European Research Council, LSHC-CT-2005-018704Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Merknad
QC 20110804Tilgjengelig fra: 2011-08-04 Laget: 2011-06-07 Sist oppdatert: 2011-12-06bibliografisk kontrollert

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