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Perfusion of an IgG producing CHO cell line at very high cell density by ATF or by TFF in WAVE Bioreactor™
KTH, Skolan för bioteknologi (BIO), Bioprocessteknik. (Cell Technology Group)
KTH, Skolan för bioteknologi (BIO), Bioprocessteknik. (Cell Technology Group)
KTH, Skolan för bioteknologi (BIO), Bioprocessteknik. (Cell Technology Group)
KTH, Skolan för bioteknologi (BIO), Bioprocessteknik. (Cell Technology Group)ORCID-id: 0000-0002-0841-8845
Vise andre og tillknytning
2011 (engelsk)Konferansepaper, Publicerat paper (Annet vitenskapelig)
Abstract [en]

Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. The properties and performances obtained in this bioreactor with both filtration systems were studied.

  • Very high cell densities were achieved and could be stably maintained at high viability indicating of a healthy process suitable for instance for efficient cell banking.

Cell density could be significantly further increased showing the capacity of the system set-up.

sted, utgiver, år, opplag, sider
2011.
Emneord [en]
perfusion, hollow fiber filter, perfusion, ATF, TFF, CHO cells
HSV kategori
Forskningsprogram
SRA - Molekylär biovetenskap
Identifikatorer
URN: urn:nbn:se:kth:diva-87442OAI: oai:DiVA.org:kth-87442DiVA, id: diva2:501653
Konferanse
HansonWade Conference Optimizing Cell Line Performance, July, 26-28, 2011, London, UK
Merknad
Invited speaker at the Hanson Wade Conference Optimizing Cell Line Performance, July, 26-28, 2011, London, UK. QC 20130319Tilgjengelig fra: 2012-02-14 Laget: 2012-02-14 Sist oppdatert: 2012-03-19bibliografisk kontrollert

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Zhang, YeChotteau, Veronique

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Clincke, Marie-FrancoiseMölleryd, CarinSamani, Puneeth K.Zhang, YeLindskog, EvaChotteau, Veronique
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