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Droplet microfluidics-A tool for single-cell analysis
KTH, Skolan för bioteknologi (BIO), Nanobioteknologi (stängd 20130101).ORCID-id: 0000-0001-5232-0805
KTH, Skolan för bioteknologi (BIO), Nanobioteknologi (stängd 20130101).
2012 (Engelska)Ingår i: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 51, nr 49, s. 12176-12192Artikel, forskningsöversikt (Refereegranskat) Published
Abstract [en]

Droplet microfluidics allows the isolation of single cells and reagents in monodisperse picoliter liquid capsules and manipulations at a throughput of thousands of droplets per second. These qualities allow many of the challenges in single-cell analysis to be overcome. Monodispersity enables quantitative control of solute concentrations, while encapsulation in droplets provides an isolated compartment for the single cell and its immediate environment. The high throughput allows the processing and analysis of the tens of thousands to millions of cells that must be analyzed to accurately describe a heterogeneous cell population so as to find rare cell types or access sufficient biological space to find hits in a directed evolution experiment. The low volumes of the droplets make very large screens economically viable. This Review gives an overview of the current state of single-cell analysis involving droplet microfluidics and offers examples where droplet microfluidics can further biological understanding. A one-off: Single-cell analysis is one of the most interesting applications for droplet microfluidics. Droplets provide robust compartments on the size scale of a single cell, and their ability to encapsulate and rapidly manipulate cells along with their immediate environment in monodisperse compartments allows the possibility of automation. This Review focuses on single-cell analyses and applications in drug screening and genetic and enzyme analysis.

Ort, förlag, år, upplaga, sidor
Wiley-VCH Verlagsgesellschaft, 2012. Vol. 51, nr 49, s. 12176-12192
Nyckelord [en]
droplets, emulsions, microfluidics, screening, single-cell analysis
Nationell ämneskategori
Biologiska vetenskaper Annan kemi
Identifikatorer
URN: urn:nbn:se:kth:diva-110171DOI: 10.1002/anie.201200460ISI: 000311706500004PubMedID: 23180509Scopus ID: 2-s2.0-84870564281OAI: oai:DiVA.org:kth-110171DiVA, id: diva2:587008
Forskningsfinansiär
VetenskapsrådetVinnovaScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Anmärkning

QC 20130114

Tillgänglig från: 2013-01-14 Skapad: 2013-01-10 Senast uppdaterad: 2019-06-11Bibliografiskt granskad

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Jönsson, Håkan N.

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Jönsson, Håkan N.Andersson Svahn, Helene
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