Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
HiRIEF LC-MSMS enables deep proteome coverage and unbiased proteogenomics
Vise andre og tillknytning
2014 (engelsk)Inngår i: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 11, nr 1, s. 59-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We present a liquid chromatography-mass spectrometry (LC-MSMS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.

sted, utgiver, år, opplag, sider
2014. Vol. 11, nr 1, s. 59-
Emneord [en]
Tandem Mass-Spectra, Peptide Identification, Human Genome, Gene, Fractionation, Spectrometry, Pseudogenes, Annotation, Landscape, Dynamics
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-140375DOI: 10.1038/NMETH.2732ISI: 000329178200027Scopus ID: 2-s2.0-84894619287OAI: oai:DiVA.org:kth-140375DiVA, id: diva2:690403
Forskningsfinansiär
Swedish Research CouncilSwedish Cancer SocietyEU, FP7, Seventh Framework Programme, GlycoHitScience for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish e‐Science Research Center
Merknad

QC 20140123

Tilgjengelig fra: 2014-01-23 Laget: 2014-01-23 Sist oppdatert: 2017-12-06bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstScopus

Personposter BETA

Käll, Lukas

Søk i DiVA

Av forfatter/redaktør
Käll, Lukas
Av organisasjonen
I samme tidsskrift
Nature Methods

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 299 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf