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Controlling acoustic streaming in a multi-well microplate for improving live cell assays
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0002-7023-4772
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0002-3996-9279
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0001-5178-7593
Vise andre og tillknytning
2011 (engelsk)Inngår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, s. 1612-1614Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.

sted, utgiver, år, opplag, sider
2011. s. 1612-1614
Emneord [en]
Acoustic streaming, Microplate, Mixing, Multi-well, Particle image velocimetry, Ultrasound, Cell trapping, Fluorescent polymers, Live cell, Micro particle image velocimetry, Microplates, Multi wells, Particle image velocimetries, Single-frequency, Flow visualization, Modulation, Ultrasonics, Velocimeters, Velocity measurement
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Identifikatorer
URN: urn:nbn:se:kth:diva-148694Scopus ID: 2-s2.0-84883821752ISBN: 9781618395955 (tryckt)OAI: oai:DiVA.org:kth-148694DiVA, id: diva2:746358
Konferanse
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Merknad

QC 20140912

Tilgjengelig fra: 2014-09-12 Laget: 2014-08-11 Sist oppdatert: 2020-01-29bibliografisk kontrollert

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Ohlin, MathiasÖnfelt, Björn

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Ohlin, MathiasChristakou, Athanasia E.Frisk, ThomasÖnfelt, BjörnWiklund, Martin
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Totalt: 190 treff
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