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Impact of the supramolecular structure of cellulose on the efficiency of enzymatic hydrolysis
KTH, School of Chemical Science and Engineering (CHE), Centres, Wallenberg Wood Science Center.ORCID iD: 0000-0001-9176-7116
2015 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 8, article id 56Article in journal (Refereed) Published
Abstract [en]

Background: The efficiency of enzymatic hydrolysis is reduced by the structural properties of cellulose. Although efforts have been made to explain the mechanism of enzymatic hydrolysis of cellulose by considering the interaction of cellulolytic enzymes with cellulose or the changes in the structure of cellulose during enzymatic hydrolysis, the process of cellulose hydrolysis is not yet fully understood. We have analysed the characteristics of the complex supramolecular structure of cellulose on the nanometre scale in terms of the spatial distribution of fibrils and fibril aggregates, the accessible surface area and the crystallinity during enzymatic hydrolysis. Influence of the porosity of the substrates and the hydrolysability was also investigated. All cellulosic substrates used in this study contained more than 96% cellulose. Results: Conversion yields of six cellulosic substrates were as follows, in descending order: nano-crystalline cellulose produced from never-dried soda pulp (NCC-OPHS-ND) > never-dried soda pulp (OPHS-ND) > dried soda pulp (OPHS-D) > Avicel > cotton treated with sodium hydroxide (cotton + NaOH) > cotton. Conclusions: No significant correlations were observed between the yield of conversion and supramolecular characteristics, such as specific surface area (SSA) and lateral fibril dimensions (LFD). A strong correlation was found between the average pore size of the starting material and the enzymatic conversion yield. The degree of crystallinity was maintained during enzymatic hydrolysis of the cellulosic substrates, contradicting previous explanations of the increasing crystallinity of cellulose during enzymatic hydrolysis. Both acid and enzymatic hydrolysis can increase the LFD, but no plausible mechanisms could be identified. The sample with the highest initial degree of crystallinity, NCC-OPHS-ND, exhibited the highest conversion yield, but this was not accompanied by any change in LFD, indicating that the hydrolysis mechanism is not based on lateral erosion.

Place, publisher, year, edition, pages
2015. Vol. 8, article id 56
Keywords [en]
Cellulose I, Enzymatic hydrolysis, Cellulose supramolecular structure, Solid-state cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS C-13-NMR), Porosity, Crystallinity
National Category
Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-166491DOI: 10.1186/s13068-015-0236-9ISI: 000352683600001PubMedID: 25870653Scopus ID: 2-s2.0-84927641545OAI: oai:DiVA.org:kth-166491DiVA, id: diva2:812342
Funder
Swedish Research Council
Note

QC 20150518

Available from: 2015-05-18 Created: 2015-05-11 Last updated: 2022-06-23Bibliographically approved

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Larsson, Per Tomas

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