We present a high-throughput technique to interface picoliter droplet microfluidics for single cell analysis with a macro scale accessible array platform by the addition of an agarose gelling agent to droplets and patterned positioning of the resulting hydrogel beads using a fluorescence activated cell sorter (FACS). This resulted in a pattern with 95 % single bead accuracy. Agarose beads containing eGFP expressing E. Coli were single sorted into microwells and E. coli growth was monitored over time.
QC 20150612