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Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
Vise andre og tillknytning
2015 (engelsk)Inngår i: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 6, artikkel-id 196Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.

sted, utgiver, år, opplag, sider
2015. Vol. 6, artikkel-id 196
Emneord [en]
T cell, IL-2, IL-7, microscopy, fluorescence, microchip, single-cell analysis, live-cell imaging
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-169286DOI: 10.3389/fimmu.2015.00196ISI: 000354937600002PubMedID: 25972868OAI: oai:DiVA.org:kth-169286DiVA, id: diva2:822640
Forskningsfinansiär
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Merknad

QC 20150617

Tilgjengelig fra: 2015-06-17 Laget: 2015-06-12 Sist oppdatert: 2017-12-04bibliografisk kontrollert

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Gustafsson, KarinGöthlin, MårtenFrisk, Thomas W.Önfelt, Björn
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