kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Compaction of rolling circle amplification products increases signal integrity and signal-to-noise ratio
Show others and affiliations
2015 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 5, article id 12317Article in journal (Refereed) Published
Abstract [en]

Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increases the local concentration of fluorophores and as a result, the signal intensity increases together with the signal-to-noise ratio. Furthermore, we have found that RCPs sometimes tend to disintegrate and may be recorded as several RCPs, a trait that is prevented with our cross-hybridizing DNA oligonucleotide. These effects generated by compaction of RCPs improve accuracy of visual as well as automated in situ analysis for RCA based methods, such as proximity ligation assays (PLA) and padlock probes.

Place, publisher, year, edition, pages
2015. Vol. 5, article id 12317
National Category
Other Biological Topics
Identifiers
URN: urn:nbn:se:kth:diva-171886DOI: 10.1038/srep12317ISI: 000358358900001PubMedID: 26202090Scopus ID: 2-s2.0-84937944116OAI: oai:DiVA.org:kth-171886DiVA, id: diva2:846074
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research Council
Note

QC 20150814

Available from: 2015-08-14 Created: 2015-08-10 Last updated: 2022-09-15Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Brismar, Hjalmar

Search in DiVA

By author/editor
Brismar, Hjalmar
By organisation
Cell PhysicsScience for Life Laboratory, SciLifeLab
In the same journal
Scientific Reports
Other Biological Topics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 436 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf