Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells.
Visa övriga samt affilieringar
2011 (Engelska)Ingår i: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 11, nr 14, s. 2432-2439Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesting cell phenotypes for further investigations. Although flow cytometry is able to sequentially probe and sort thousands of cells per second, dynamic processes cannot be experimentally accessed on single cells due to the sub-second sampling time. Cellular dynamics can be measured by image cytometry of surface-immobilized cells, however, cell sorting is complicated under these conditions due to cell attachment. We here developed a cytometric tool based on refractive multiple optical tweezers combined with microfluidics and optical microscopy. We demonstrate contact-free immobilization of more than 200 yeast cells into a high-density array of optical traps in a microfluidic chip. The cell array could be moved to specific locations of the chip enabling us to expose in a controlled manner the cells to reagents and to analyze the responses of individual cells in a highly parallel format using fluorescence microscopy. We further established a method to sort single cells within the microfluidic device using an additional steerable optical trap. Ratiometric fluorescence imaging of intracellular pH of trapped yeast cells allowed us on the one hand to measure the effect of the trapping laser on the cells' viability and on the other hand to probe the dynamic response of the cells upon glucose sensing.

Ort, förlag, år, upplaga, sidor
Royal Society of Chemistry, 2011. Vol. 11, nr 14, s. 2432-2439
Nyckelord [en]
yeast saccharomyces-cerevisiae, single-cell, intracellular PH, flow-cytometry, gene-expression, biological cells, mammalian-cells, manipulation, growth, noise
Nationell ämneskategori
Biofysik Analytisk kemi Atom- och molekylfysik och optik
Identifikatorer
URN: urn:nbn:se:kth:diva-178096DOI: 10.1039/c1lc20181fISI: 000292168500018PubMedID: 21655617Scopus ID: 2-s2.0-80051757580OAI: oai:DiVA.org:kth-178096DiVA, id: diva2:877622
Anmärkning

QC 2016-01-18

Tillgänglig från: 2015-12-07 Skapad: 2015-12-07 Senast uppdaterad: 2017-12-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Piguet, Joachim

Sök vidare i DiVA

Av författaren/redaktören
Piguet, Joachim
I samma tidskrift
Lab on a Chip
BiofysikAnalytisk kemiAtom- och molekylfysik och optik

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 88 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf