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Solid tumor spheroid formation by temperature-controlled high voltage ultrasound in a multi-well microdevice
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0002-7023-4772
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.ORCID-id: 0000-0001-5178-7593
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
2014 (engelsk)Inngår i: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems SocietyChemical and Biological Microsystems Society , 2014, s. 573-575Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

In the present work we demonstrate effective 3D growth of human hepatocellular carcinoma (HCC) HepG2 cell spheroids in parallel in a multi-well microdevice actuated with high voltage ultrasound in a temperature-controlled system. We compare the spheroid formation during continuous ultrasound exposure for one week where we formed spheroids in 59% of the wells, with the spheroid formation without ultrasound actuation, where we obtained 0% spheroids. Furthermore, we present an application of the tumor spheroids for investigating natural killer (NK) cells behavior against solid tumors.

sted, utgiver, år, opplag, sider
Chemical and Biological Microsystems SocietyChemical and Biological Microsystems Society , 2014. s. 573-575
Emneord [en]
Microchip, Multi-well, Natural killer cells, Solid tumor, Spheroid, Ultrasound, Temperature control, Tumors, Multi wells, Solid tumors, Ultrasonics
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-174813Scopus ID: 2-s2.0-84941633758ISBN: 9780979806476 (tryckt)OAI: oai:DiVA.org:kth-174813DiVA, id: diva2:881498
Konferanse
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
Merknad

QC 20151210

Tilgjengelig fra: 2015-12-10 Laget: 2015-10-07 Sist oppdatert: 2018-01-10bibliografisk kontrollert

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Ohlin, MathiasÖnfelt, Björn

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Totalt: 413 treff
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