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Single-cell screening of photosynthetic growth and lactate production by cyanobacteria
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab. (Novo Nordisk Foundation Center for Biosustainability)
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab. (Novo Nordisk Foundation Center for Biosustainability)
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
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2015 (Engelska)Ingår i: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 8, artikel-id 193Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

Background: Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible. Results: We present a method for high-throughput, single-cell analysis and sorting of genetically engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for L-lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive L-lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells. Conclusions: The workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology.

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BioMed Central, 2015. Vol. 8, artikel-id 193
Nyckelord [en]
Cyanobacteria, Single cell, Droplet microfluidics, High-throughput screening, Lactate dehydrogenase
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:kth:diva-180126DOI: 10.1186/s13068-015-0380-2ISI: 000365784000009Scopus ID: 2-s2.0-84947929588OAI: oai:DiVA.org:kth-180126DiVA, id: diva2:894074
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QC 20160114

Tillgänglig från: 2016-01-14 Skapad: 2016-01-07 Senast uppdaterad: 2017-11-30Bibliografiskt granskad

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Hudson, Elton P.Jönsson, Håkan N.

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Hammar, PetterSjöström, Staffan L.van der Meer, JosefinHudson, Elton P.Jönsson, Håkan N.
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Proteomik och nanobioteknologiScience for Life Laboratory, SciLifeLab
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Biotechnology for Biofuels
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