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2015 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 37, no 4, p. 825-830Article in journal (Refereed) Published
Abstract [en]
Blood-stream infections (BSI) remain a major health challenge, with an increasing incidence worldwide and a high mortality rate. Early treatment with appropriate antibiotics can reduce BSI-related morbidity and mortality, but success requires rapid identification of the infecting organisms. The rapid, culture-independent diagnosis of BSI could be significantly facilitated by straightforward isolation of highly purified bacteria from whole blood. We present a microfluidic-based, sample-preparation system that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100 % viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification.
Keywords
Bacteria; Cells; Cytology; Deionized water; Microfluidics, Bacteria isolation; Blood streams; Cell lysis; Drug susceptibility; Isolation of bacteria; Microfluidic-based; Molecular identification; Rapid identification, Blood
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-157710 (URN)10.1007/s10529-014-1734-8 (DOI)000351535300010 ()25413883 (PubMedID)2-s2.0-84925535660 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20141212
2014-12-122014-12-122024-03-18Bibliographically approved