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The Gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles
Dep. of Biochemistry, Max Planck institute for Chemical Ecology.
KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi. (Ekologisk kemi)ORCID-id: 0000-0003-4970-8352
Dep. of Ecology, SLU.
Max Planck Institute for Chemical Ecology. (Dep of Biochemistry)
Visa övriga samt affilieringar
2016 (Engelska)Ingår i: Molecular Ecology, ISSN 0962-1083, E-ISSN 1365-294X, Vol. 25, nr 16, s. 4014-4031Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The pine weevil (Hylobius abietis, Coleoptera: Curculionidae) is an important pest of conifer seedlings in Europe. Despite its economic importance, little is known about the composition of its gut microbial community and the role it plays in mediating the weevil's ability to utilize conifers as a food source. Here, we characterized the gut bacterial communities of different populations of H. abietis across Europe and compared them to those of other beetles that occupy similar ecological niches. We demonstrate that the microbial community of H. abietis is similar at higher taxonomic levels (family and genus) across locations in Europe, with Wolbachia as the dominant microbe, followed by Enterobacteria and Firmicutes. Despite this similarity, we observed consistent differences between countries and locations, but not sexes. Our meta-analysis demonstrates that the gut bacterial community of the pine weevil is very similar to that of bark beetles that also exploit conifers as a food source. The Enterobacteriaceae symbionts of both host taxa are especially closely related phylogenetically. Conversely, the microbiota of H. abietis is distinct from that of closely related weevils feeding on non-conifer food sources, suggesting that the microbial community of the pine weevil is determined by the environment and may be relevant to host ecology. Furthermore, several H. abietis-associated members of the Enterobacteriaceae family are known to contain genes involved in terpenoid degradation. As such, we hypothesize that the gut microbial community is important for the utilization of conifer seedlings as a food source, either through the detoxification of plant secondary metabolites or supplementation of essential nutrients.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2016. Vol. 25, nr 16, s. 4014-4031
Nyckelord [en]
Hylobius abietis, conifer, terpenes, insect symbiosis, bark beetle, microbiota
Nationell ämneskategori
Mikrobiologi Ekologi
Forskningsämne
Kemi
Identifikatorer
URN: urn:nbn:se:kth:diva-187816DOI: 10.1111/mec.13702ISI: 000381578200017Scopus ID: 2-s2.0-84981710086OAI: oai:DiVA.org:kth-187816DiVA, id: diva2:931673
Anmärkning

QC 20160530

Tillgänglig från: 2016-05-30 Skapad: 2016-05-30 Senast uppdaterad: 2017-11-30Bibliografiskt granskad
Ingår i avhandling
1. Chemical signals in interactions between Hylobius abietis and associated bacteria
Öppna denna publikation i ny flik eller fönster >>Chemical signals in interactions between Hylobius abietis and associated bacteria
2016 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The pine weevil (Hylobius abietis L.) is one of the two topmost economically important insect pests in Swedish conifer forests. The damage increase in areas were the silvicultural practice is to use clear cuttings were the insects gather and breed. During egglaying the female protects her offspring by creating a cave in roots and stumps were she puts her egg and covers it with frass, a mixture of weevil feces and chewed bark. Adult pine weevils have been observed to feed on the other side of the egg laying site and antifeedant substance has been discovered in the feces of the pine weevil. We think it is possible that microorganisms present in the frass contribute with antifeedant/repellent substances. Little is known about the pine weevils associated bacteria community and their symbiotic functions. In this thesis the bacterial community is characterized in gut and frass both from pine weevils in different populations across Europe as well as after a 28 day long diet regime on Scots pine, silver birch or bilberry. Volatile substances produced by isolated bacteria as well as from a consortium of microorganisms were collected with solid phase micro extraction (SPME) and analyzed with GC-MS. The main volatiles were tested against pine weevils using a two-choice test. Wolbachia, Rahnella aquatilis, Serratia and Pseudomonas syringae was commonly associated with the pine weevil. 2-Methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol were found in the headspace from Rahnella aquatilis when grown in substrate containing pine bark. 2-Methoxyphenol and 3-methyl-1-butanol, phenol and methyl salicylate were found in pine feces. Birch and bilberry feces emitted mainly linalool oxides and bilberry emitted also small amounts of 2-phenylethanol.

A second part of the thesis discusses the role of fungi in forest insect interactions and the production of oxygenated monoterpenes as possible antifeedants. Spruce bark beetles (Ips typhographus L.) aggregate with the help of pheromones and with collected forces they kill weakened adult trees as a result of associated fungi growth and larval development. A fungi associated with the bark beetle, Grosmannia europhoides, was shown to produce de novo 2-methyl-3-buten-2-ol, the major component of the spruce bark beetle aggregation pheromone. Chemical defense responses against Endoconidiophora polonica and Heterobasidion parviporum were investigated using four clones of Norway spruce with different susceptibility to Heterobasidion sp. Clone specific differences were found in induced mono-, sesqui and diterpenes. A number of oxygenated monoterpenes which are known antifeedants for the pine weevil were produced in the infested areas.

Ort, förlag, år, upplaga, sidor
Stockholm: Kungliga tekniska högskolan, 2016. s. 51
Serie
TRITA-CHE-Report, ISSN 1654-1081 ; 21
Nyckelord
Pine weevil, Hylobius, Rahnella, Pseudomonas, bacteria, fungi, metabolites, 2-methoxyphenol, 2-phenylethanol
Nationell ämneskategori
Analytisk kemi Ekologi Mikrobiologi Biokemi och molekylärbiologi Organisk kemi
Forskningsämne
Kemi
Identifikatorer
urn:nbn:se:kth:diva-187817 (URN)978-91-7595-972-6 (ISBN)
Disputation
2016-06-15, F3, Lindstedtvägen 26, Stockholm, 12:00 (Engelska)
Opponent
Handledare
Forskningsfinansiär
Forskningsrådet FormasStiftelsen för strategisk forskning (SSF)
Anmärkning

QC 20160601

Tillgänglig från: 2016-05-30 Skapad: 2016-05-30 Senast uppdaterad: 2016-06-01Bibliografiskt granskad

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Förlagets fulltextScopusMolecular ecology

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Axelsson, Karolin

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