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Antiplasmodial activity and phytochemical analysis of extracts from selected Ugandan medicinal plants
Vise andre og tillknytning
2016 (engelsk)Inngår i: Journal of Ethnopharmacology, ISSN 0378-8741, E-ISSN 1872-7573, Vol. 186, s. 14-19Artikkel i tidsskrift (Fagfellevurdert) Published
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Abstract [en]

Ethnopharmacological relevance: Resistance of the parasites to known antimalarial drugs has provided the necessity to find new drugs from natural products against malaria. The aim of the study was to evaluate the in vitro antiplasmodial activity of some plants used by Traditional Medical Practitioners (TMPs) of Prometra and Rukararwe in malaria treatment in Uganda to provide scientific proof of the efficacies claimed by these Herbalists. Materials and methods: The air dried samples of Clerodendrum rotundifolium (leaves), Microglossa pyrifolia (leaves), Momordica foetida (leaves) and Zanthoxylum chalybeum (stem bark) used for malaria treatment by TMPs were successively extracted with ethyl acetate, methanol and water to yield twelve extracts. The extracts were tested against the chloroquine-sensitive (NF54) and chloroquine-resistant (FCR3) Plasmodium falciparum strains in vitro using the micro Mark III test which is based on assessing the inhibition of schizont maturation. A compound A was extracted and purified from the stem bark of Z. chalybeum and its structure was identified and confirmed by spectroscopic methods. Results: Most of the extracts tested (92%) showed an antiplasmodial activity with IC50 < 50 mu g/mL. In spite of successive extractions with different solvents, potent anti-plasmodial activity (IC50 < 5 mu g/mL) was observed in the ethyl acetate, methanol and aqueous extracts of M. pyrifolia and C. rotundifolium. Preferential enrichments of activity into water (IC50 < 15 mu g/mL) and Ethyl acetate (IC50 < 5 mu g/mL) were seen in the case of M. foetida and Z chalybeum respectively. The most active extracts were from C rotundifolium and M. pyrifolia with IC50 values less than 2 mu g/mL. Phytochemical analysis of the extracts revealed the presence of saponins, tannins, flavonoids, alkaloids and cardiac glycocides. Fagaramide isolated from Z chalybeum had a higher activity (IC50 2.85 mu g/mL) against the chloroquine-resistant strain than against the chloroquine-senstive (IC50 16.6 mu g/mL) strain used in the study. Conclusion: The plant extracts analysed in this study presented an average antiplasmodial activity (58%). This study revealed for the first time the antiplasmodial activity of the plant C. rotundofolium. It's the first time the compound fagaramide (N-isobutyl-3-(3,4-methylene dioxyphenyl) - 2E-propenamide) has been isolated from Z. chalybeum as one of the compounds that contribute to the activity of this plant against P. falciparum.

sted, utgiver, år, opplag, sider
Elsevier, 2016. Vol. 186, s. 14-19
Emneord [en]
Antiplasmodial activity, Plasmodium falciparum, Clerodendrum rotundifolium, Microglossa pyrifolia, Momordica foetida, Zanthoxylum chalybeum
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Identifikatorer
URN: urn:nbn:se:kth:diva-189660DOI: 10.1016/j.jep.2016.03.047ISI: 000377832000002PubMedID: 27019273Scopus ID: 2-s2.0-84977610683OAI: oai:DiVA.org:kth-189660DiVA, id: diva2:949013
Forskningsfinansiär
Sida - Swedish International Development Cooperation Agency
Merknad

QC 20160715

Tilgjengelig fra: 2016-07-15 Laget: 2016-07-11 Sist oppdatert: 2017-11-28bibliografisk kontrollert

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