Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
High density of REC8 constrains sister chromatid axes and prevents illegitimate synaptonemal complex formation
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0002-4720-2756
Vise andre og tillknytning
2016 (engelsk)Inngår i: EMBO Reports, ISSN 1469-221X, E-ISSN 1469-3178, Vol. 17, nr 6, s. 901-913Artikkel i tidsskrift (Fagfellevurdert) Published
Resurstyp
Text
Abstract [en]

During meiosis, cohesin complexes mediate sister chromatid cohesion (SCC), synaptonemal complex (SC) assembly and synapsis. Here, using super-resolution microscopy, we imaged sister chromatid axes in mouse meiocytes that have normal or reduced levels of cohesin complexes, assessing the relationship between localization of cohesin complexes, SCC and SC formation. We show that REC8 foci are separated from each other by a distance smaller than 15% of the total chromosome axis length in wild-type meiocytes. Reduced levels of cohesin complexes result in a local separation of sister chromatid axial elements (LSAEs), as well as illegitimate SC formation at these sites. REC8 but not RAD21 or RAD21L cohesin complexes flank sites of LSAEs, whereas RAD21 and RAD21L appear predominantly along the separated sister-chromatid axes. Based on these observations and a quantitative distribution analysis of REC8 along sister chromatid axes, we propose that the high density of randomly distributed REC8 cohesin complexes promotes SCC and prevents illegitimate SC formation.

sted, utgiver, år, opplag, sider
Wiley-VCH Verlagsgesellschaft, 2016. Vol. 17, nr 6, s. 901-913
Emneord [en]
cohesin, meiosis, sister chromatid cohesion, super-resolution microscopy, synaptonemal complex
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-189673DOI: 10.15252/embr.201642030ISI: 000377707400014PubMedID: 27170622Scopus ID: 2-s2.0-84970005648OAI: oai:DiVA.org:kth-189673DiVA, id: diva2:949245
Forskningsfinansiär
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Cancer SocietySwedish Research Council
Merknad

QC 20160718

Tilgjengelig fra: 2016-07-18 Laget: 2016-07-11 Sist oppdatert: 2017-11-28bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Søk i DiVA

Av forfatter/redaktør
Manneberg, OttoBlom, HansBrismar, Hjalmar
Av organisasjonen
I samme tidsskrift
EMBO Reports

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 1082 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf