Reversible Sulfur Reactions in Pre-Equilibrated and Catalytic Self-Screening Dynamic Combinatorial Chemistry Protocols
2006 (English)Licentiate thesis, comprehensive summary (Other scientific)
Dynamic Combinatorial Chemistry (DCC) is a recently introduced supramolecular approach to generate dynamically interchanging libraries of compounds. These libraries are made of different building blocks that reversibly interact with one another and spontaneously assemble to encompass all possible combinations. If a target molecule, for instance a receptor is added to the system and one or more molecules show affinity to the target species, these compounds will, according to Le Châtelier´s principle, be amplified on the expense of the other non-bonding constituents. To date, only a handful of different systems and formats have been used. Hence, to further advance the technique, especially when biological systems are targeted, new reaction types and new screening methods are necessary. This thesis describes the development of reversible sulfur reactions, thiol/disulfide interchange and transthiolesterification (the latter being a new reaction type for DCC), as means of generating reversible covalent bond reactions. Two different types of target proteins are used, enzymes belonging to the hydrolase family and the plant lectin Concanavalin A. Furthermore, two new screening/analysis methods not previously used in DCC are also presented; the quartz crystal microbalance (QCM)-technique and catalytic self-screening.
Place, publisher, year, edition, pages
Stockholm: KTH , 2006. , 44 p.
Trita-IOK, ISSN 1100-7974 ; 2006:102
Dynamic Combinatorial Chemistry, Reversible sulfur reactions, Catalytic self-screening, Carbohydrates, Lectins, Quartz Crystal Microbalance
IdentifiersURN: urn:nbn:se:kth:diva-3917ISBN: 91-7178-323-7OAI: oai:DiVA.org:kth-3917DiVA: diva2:10008
2006-05-02, F3, Lindstedtsvägen 26, KTH, Stockholm, 10:00
QC 201011182006-04-102006-04-102010-11-18Bibliographically approved
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