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Rapid PCR amplification of DNA utilizing Coriolis effects
KTH, School of Engineering Sciences (SCI), Mechanics.
KTH, School of Engineering Sciences (SCI), Mechanics.
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2006 (English)In: European Biophysics Journal, ISSN 0175-7571, E-ISSN 1432-1017, Vol. 35, no 6, 453-458 p.Article in journal (Refereed) Published
Abstract [en]

A novel polymerase chain reaction (PCR) method is presented that utilizes Coriolis and centrifugal effects, produced by rotation of the sample disc, in order to increase internal circulatory rates, and with them temperature homogenization and mixing speeds. A proof of concept has been presented by testing a rapid 45-cycle PCR DNA amplification protocol. During the repeated heating and cooling that constitutes a PCR process, the 100 mu L samples were rotated at a speed equivalent to an effective acceleration of gravity of 7,000 g. A cycle time of 20.5 s gave a total process time of 15 min to complete the 45 cycles. A theoretical and numerical analysis of the resulting flow, which describes the increased mixing and temperature homogenization, is presented. The device gives excellent reaction speed efficiency, which is beneficial for rapid PCR.

Place, publisher, year, edition, pages
2006. Vol. 35, no 6, 453-458 p.
National Category
Fluid Mechanics and Acoustics
Identifiers
URN: urn:nbn:se:kth:diva-5623DOI: 10.1007/s00249-006-0052-zISI: 000238860400001Scopus ID: 2-s2.0-33745806547OAI: oai:DiVA.org:kth-5623DiVA: diva2:10051
Note
QC 20100913Available from: 2006-04-27 Created: 2006-04-27 Last updated: 2010-09-13Bibliographically approved
In thesis
1. Analysis of laminar and turbulent flows with turbomachinery, biotechnology and biomechanical applications
Open this publication in new window or tab >>Analysis of laminar and turbulent flows with turbomachinery, biotechnology and biomechanical applications
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

The goal of this study was initially to gain a better understanding of the effects of rotation on turbulent flow in ducts. Knowledge concerning the influence of rotation on the structures of turbulence is of fundamental importance in many applications, e.g. centrifugal separators, turbines or cooling channels in rotating machinery, as well as meteorology and oceanography. Rapidly rotating duct flow is studied experimentally with rotation numbers in the interval [ 0, 1] . To achieve this, in combination with relatively high Reynolds numbers (5000 – 30000 based on the hydraulic radius), water was used as the working medium. The influence of the rotation on the pressure drop in the duct was investigated and suitable scalings of this quantity were studied. Due to questions that arose in the experimental study, two numerical studies were initiated. The first study probed the effect of rotation and geometrical configuration on the development length for turbulent flow, while the second comprised a direct numerical simulation of turbulent flow in a rotating duct. It is shown that while system rotation does not have a marked effect on the development length in a plane channel, the development length is substantially shortened in a duct.

Additional systems subject to rotation or curvature effects were studied. The laminar flow of fluid in a rotating PCR-cone was analysed analytically and numerically to understand the increased mixing and temperature homogenization. The flow field in the cone was described and the increased mixing was due to a strong boundary layer flow incited by Coriolis and buoyancy effects. Comparisons of the numerical simulations with experiments yielded good results.

A study to quantify the flow of blood in cerebral malformations using three-dimensional videodensitometry was performed. Data from experiments with an idealized flow phantom, as well clinical pathologies, showed that the proposed methodology in conjunction with clinical injection protocols can yield mean flux data with an error less than 20%. Protocol improvements are proposed.

Place, publisher, year, edition, pages
Stockholm: Mekanik, 2006
Series
Trita-MEK, ISSN 0348-467X ; 2006:06
National Category
Fluid Mechanics and Acoustics
Identifiers
urn:nbn:se:kth:diva-3928 (URN)
Public defence
2006-05-05, Sal F2, Lindstedtsvägen 28, Stockholm, 13:30
Opponent
Supervisors
Available from: 2006-04-27 Created: 2006-04-27 Last updated: 2012-03-21

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Johansson, Arne V.

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