Rapid PCR amplification of DNA utilizing Coriolis effects
2006 (English)In: European Biophysics Journal, ISSN 0175-7571, E-ISSN 1432-1017, Vol. 35, no 6, 453-458 p.Article in journal (Refereed) Published
A novel polymerase chain reaction (PCR) method is presented that utilizes Coriolis and centrifugal effects, produced by rotation of the sample disc, in order to increase internal circulatory rates, and with them temperature homogenization and mixing speeds. A proof of concept has been presented by testing a rapid 45-cycle PCR DNA amplification protocol. During the repeated heating and cooling that constitutes a PCR process, the 100 mu L samples were rotated at a speed equivalent to an effective acceleration of gravity of 7,000 g. A cycle time of 20.5 s gave a total process time of 15 min to complete the 45 cycles. A theoretical and numerical analysis of the resulting flow, which describes the increased mixing and temperature homogenization, is presented. The device gives excellent reaction speed efficiency, which is beneficial for rapid PCR.
Place, publisher, year, edition, pages
2006. Vol. 35, no 6, 453-458 p.
Fluid Mechanics and Acoustics
IdentifiersURN: urn:nbn:se:kth:diva-5623DOI: 10.1007/s00249-006-0052-zISI: 000238860400001ScopusID: 2-s2.0-33745806547OAI: oai:DiVA.org:kth-5623DiVA: diva2:10051
QC 201009132006-04-272006-04-272010-09-13Bibliographically approved