Ultra-High-Throughput Sample Preparation System for Lymphocyte Immunophenotyping Point-of-Care Diagnostics
2016 (English)In: JALA, ISSN 2211-0682, Vol. 21, no 5, 706-712 p.Article in journal (Refereed) Published
Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples. Utilizing commercialization-friendly manufacturing methods and materials, yielding a sample throughput of 20 mL/min, and allowing for on-chip staining and imaging bring together a practical, yet powerful approach to microfluidic diagnostics of large, dilute samples.
Place, publisher, year, edition, pages
Sage Publications, 2016. Vol. 21, no 5, 706-712 p.
centrifugal microfluidics, paper-based microfluidics, cell capture, sample preparation, cell immunophenotyping
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-194261DOI: 10.1177/2211068216634003ISI: 000384462700010PubMedID: 26920576ScopusID: 2-s2.0-84988640558OAI: oai:DiVA.org:kth-194261DiVA: diva2:1040748
QC 201610282016-10-282016-10-212016-11-03Bibliographically approved