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Ultra-High-Throughput Sample Preparation System for Lymphocyte Immunophenotyping Point-of-Care Diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. Northeastern University, United States.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
2016 (English)In: JALA, ISSN 2211-0682, Vol. 21, no 5, 706-712 p.Article in journal (Refereed) Published
Abstract [en]

Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples. Utilizing commercialization-friendly manufacturing methods and materials, yielding a sample throughput of 20 mL/min, and allowing for on-chip staining and imaging bring together a practical, yet powerful approach to microfluidic diagnostics of large, dilute samples.

Place, publisher, year, edition, pages
Sage Publications, 2016. Vol. 21, no 5, 706-712 p.
Keyword [en]
centrifugal microfluidics, paper-based microfluidics, cell capture, sample preparation, cell immunophenotyping
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-194261DOI: 10.1177/2211068216634003ISI: 000384462700010PubMedID: 26920576ScopusID: 2-s2.0-84988640558OAI: oai:DiVA.org:kth-194261DiVA: diva2:1040748
Note

QC 20161028

Available from: 2016-10-28 Created: 2016-10-21 Last updated: 2016-11-03Bibliographically approved

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Walsh, David I., IIIRussom, Aman
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