S18 family of mitochondrial ribosomal proteins: Evolutionary history and Gly132 polymorphism in colon carcinoma
2016 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 7, no 34, 55649-55662 p.Article in journal (Refereed) Published
S18 family of mitochondrial ribosomal proteins (MRPS18, S18) consists of three members, S18-1 to -3. Earlier, we found that overexpression of S18-2 protein resulted in immortalization and eventual transformation of primary rat fibroblasts. The S18-1 and -3 have not exhibited such abilities. To understand the differences in protein properties, the evolutionary history of S18 family was analyzed. The S18-3, followed by S18-1 and S18-2 emerged as a result of ancient gene duplication in the root of eukaryotic species tree, followed by two metazoan-specific gene duplications. However, the most conserved metazoan S18 homolog is the S18-1; it shares the most sequence similarity with S18 proteins of bacteria and of other eukaryotic clades. Evolutionarily conserved residues of S18 proteins were analyzed in various cancers. S18-2 is mutated at a higher rate, compared with S18-1 and -3 proteins. Moreover, the evolutionarily conserved residue, Gly132 of S18-2, shows genetic polymorphism in colon adenocarcinomas that was confirmed by direct DNA sequencing. Concluding, S18 family represents the yet unexplored important mitochondrial ribosomal proteins.
Place, publisher, year, edition, pages
Impact journals , 2016. Vol. 7, no 34, 55649-55662 p.
Evolutionary trace analysis, Genetic polymorphism, Mitochondrial ribosomal proteins, MRPS18 family, Phylogenetic analysis
Computer and Information Science
IdentifiersURN: urn:nbn:se:kth:diva-194597DOI: 10.18632/oncotarget.10957ScopusID: 2-s2.0-84983489932OAI: oai:DiVA.org:kth-194597DiVA: diva2:1044132
Correspondence Address: Kashuba, E.; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska InstituteSweden; email: Elena.Kashuba@ki.se. QC 201611022016-11-022016-10-312016-11-02Bibliographically approved