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Specific Binding of Cu(II) Ions to Amyloid-Beta Peptides Bound to Aggregation-Inhibiting Molecules or SDS Micelles Creates Complexes that Generate Radical Oxygen Species
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2016 (English)In: Journal of Alzheimer's Disease, ISSN 1387-2877, E-ISSN 1875-8908, Vol. 54, no 3, 971-982 p.Article in journal (Refereed) Published
Abstract [en]

Aggregation of the amyloid-beta (Aβ) peptide into insoluble plaques is a major factor in Alzheimer's disease (AD) pathology. Another major factor in AD is arguably metal ions, as metal dyshomeostasis is observed in AD patients, metal ions modulate Aβ aggregation, and AD plaques contain numerous metals including redox-active Cu and Fe ions. In vivo, Aβ is found in various cellular locations including membranes. So far, Cu(II)/Aβ interactions and ROS generation have not been investigated in a membrane environment. Here, we study Cu(II) and Zn(II) interactions with Aβ bound to SDS micelles or to engineered aggregation-inhibiting molecules (the cyclic peptide CP-2 and the Z Aβ3 (12-58)Y18L Affibody molecule). In all studied systems the Aβ N-terminal segment was found to be unbound, unstructured, and free to bind metal ions. In SDS micelles, Aβ was found to bind Cu(II) and Zn(II) with the same ligands and the same K D as in aqueous solution. ROS was generated in all Cu(II)/Aβ complexes. These results indicate that binding of Aβ to membranes, drugs, and other entities that do not interact with the Aβ N-terminal part, appears not to compromise the N-terminal segment's ability to bind metal ions, nor impede the capacity of N-terminally bound Cu(II) to generate ROS. © 2016-IOS Press and the authors. All rights reserved.

Place, publisher, year, edition, pages
IOS Press, 2016. Vol. 54, no 3, 971-982 p.
Keyword [en]
Alzheimer's disease, copper-binding protein, hydrogen peroxide, membrane chemistry, neurodegeneration, protein aggregation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-194596DOI: 10.3233/JAD-160427ScopusID: 2-s2.0-84990053593OAI: oai:DiVA.org:kth-194596DiVA: diva2:1044137
Note

Correspondence Address: Wärmländer, S.K.T.S.; Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm UniversitySweden; email: seb@dbb.su.se, QC 20161102

Available from: 2016-11-02 Created: 2016-10-31 Last updated: 2016-11-02Bibliographically approved

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