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Sökande efter nya enzymer för användning vid förbehandling av lignocellulosa
KTH, School of Biotechnology (BIO).
2016 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
In search of novel enzymes for use in pre-treatment of lignocellulosic biomass (English)
Abstract [en]

The impact of carbon-dioxide emission on the environment is one of our generation’sbiggest environmental challenges, and the use of more sustainable energy sources isneeded. Bioethanol can act as a more sustainable resource instead of petroleum basedfuels. The biggest obstacle during production of second-generation bioethanol is thepre-treatment step, where the lignin is removed from the cellulolytic material.Laccases are enzymes that have the ability to oxidise lignin and thus remove ligninfrom the material. Recent articles reporting on enzymes belonging to the proteinfamily DUF152 have shown that they possess a laccase-like activity. Therefor,members of the DUF152 family are interesting targets for possible future applicationsthat involve pre-treatment of cellulolytic materials. In this report structural andpreliminary biochemical studies are presented for several novel DUF152 membersreferred to as B05, B10 and Dv152.

The proteins were produced in Escherichia coli cells and purified by immobilisedmetal ion affinity chromatography and size exclusion chromatography. Crystallisationexperiments were successful and yielded high-resolution diffraction data for all threeenzymes. The crystal structures were determined at 2.10, 2.30 and 1.60 Å resolutionfor B05 was 2.1 Å, B10 2.3 Å and for Dv152 1.6 Å respectively. The amino acids inthe active site were shown to be conserved and included two catalytic 2 histidineresidues and one cysteine residue. To further evauate similarities and differences ofstructural features, homology models were also generated for DUF152 members thathave been biochemically characterised but where no crystal structure is available.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Second-generation biofuel, biomass, enzyme pre-treatment, laccase, protein production, enzyme assay, crystal structure
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-190744OAI: oai:DiVA.org:kth-190744DiVA: diva2:1044163
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Available from: 2017-09-11 Created: 2016-08-15 Last updated: 2017-09-11Bibliographically approved

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf