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Östrogen-medierad signalering och effekt i kolonepitelceller
KTH, School of Biotechnology (BIO).
2016 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Exploring the estrogen signaling pathway and impact in colon epithelial cells (English)
Abstract [en]

Several epidemiological, in vitro and animal studies using full ERβ knockout(fERβKO) mice suggest that ERβ can be a possible target for colorectal cancer (CRC) prevention andtreatment but the mechanisms are not fully understood. In the present study, intestine specific ERβknockout (iERβKO) mice lacking ERβ specifically in the intestinal epithelial cells have been comparedto mice expressing ERβ, and fERβKO mice completely lacking ERβ, before and after azoxymethane(AOM)/dextran sodium sulfate (DSS) carcinogenic treatment. Alcian blue staining, which specificallystains goblet cells, immunohistochemistry (IHC) with an antibody against the proliferative markerBromodeoxyuridine (BrdU), quantitative PCR (qPCR) and an enzyme-linked immunosorbent assay(ELISA) for the detection of inflammatory markers have been performed. The percentage ofproliferative cells increased as a result of intestinal loss of ERβ in the AOM/DSS model and thenumber of goblet cells did show a trend of increase for the fERβKO mice. These results indicate thatERβ in colon epithelial cells is important for cell proliferation/cancer progression and ERβ in othercells, e.g. immune cells is important for inflammation.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Colorectal cancer, ERβ, Estrogen receptor, mouse model, intestine specific ERβ knockout, iERβKO, AOM/DSS, Alcian blue, goblet cells, IHC, BrdU, qPCR
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-190594OAI: oai:DiVA.org:kth-190594DiVA: diva2:1044172
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Available from: 2017-09-11 Created: 2016-08-12 Last updated: 2017-09-11Bibliographically approved

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  • apa
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