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Comparison between 10 000 year old and contemporary spruce lignin
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
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2009 (English)In: Wood Science and Technology, ISSN 0043-7719, E-ISSN 1432-5225, Vol. 43, no 1-2, 23-41 p.Article in journal (Refereed) Published
Abstract [en]

 Wood from white spruce Picea glauca that had been preserved by rapid burial in lake sediments 10,000 years ago, was investigated and compared to a contemporary reference white spruce wood. The 10,000-year old sample appeared to have an intact primary cell wall and middle lamella, whereas the carbohydrate monomer distribution, and microscopic images showed that the secondary wall was at least partially removed, indicating that this structure had been selectively attacked by bacteria. The Klason lignin amount in the aged spruce was found to be 60%. The relative lignin monomer content in the aged spruce was 9% lower than that of the reference wood, showing that there were fewer beta-O-4' linkages in the aged sample. This finding was supported by SEC analysis of the thioacidolysed samples as a larger proportion of lignin oligomers were observed in the aged spruce than in the reference material. This indicates a somewhat greater number of condensed bonds in the aged spruce than in the reference spruce sample. Quantitative C-13 NMR analysis and HSQC techniques applied on milled wood lignins (MWL) revealed no significant structural differences between the aged spruce and the reference.

Place, publisher, year, edition, pages
2009. Vol. 43, no 1-2, 23-41 p.
Keyword [en]
Carbohydrates, Gel permeation chromatography, Monomers, Oligomers, Polymers, Walls (structural partitions), Wood
National Category
Paper, Pulp and Fiber Technology
Identifiers
URN: urn:nbn:se:kth:diva-5948DOI: 10.1007/s00226-008-0222-5ISI: 000263392100003Scopus ID: 2-s2.0-60649094082OAI: oai:DiVA.org:kth-5948DiVA: diva2:10494
Note
QC 20100903. Uppdaterat från Manuskript till Artikel (20100903) Available from: 2006-06-02 Created: 2006-06-02 Last updated: 2010-09-20Bibliographically approved
In thesis
1. Composition of lignin in outer cell-wall layers
Open this publication in new window or tab >>Composition of lignin in outer cell-wall layers
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. Accurate data relating to the lignin monomer composition and the number of β-O-4´ bonds were obtained from pure middle lamella/primary cell wall lignin. Firstly, a 10 000 year old white spruce material, with most of the secondary cell wall missing, was studied. The aged lignin was composed of guaiacyl units only, and was slightly more condensed but otherwise similar to the reference lignin.

Secondly, the developing xylem of a Norway spruce clone was analyzed during a growth season. In spring and early summer, growth is very rapid and the intention was to sample tissues in which the secondary cell-wall layers had not yet lignified, but where the outer layers at least had started to lignify. Microscopy, Klason lignin and carbohydrate analyses showed that the lignin in the developing xylem of samples from mid-June was located exclusively in the middle lamella. The lignin was more condensed, was composed of guaiacyl units only and contained more end-groups than the reference Norway spruce wood.

Thirdly, the cambial tissues of a Balsam poplar clone were surveyed during a growth season. Both the phloem side and the xylem side of the cambial region were examined. The Klason lignin content and carbohydrate monomer distribution showed that in June and August the tissues on the phloem side contained material with mainly middle lamella/primary walls. In June, the xylem side in the cambial region contained mainly middle lamella/primary walls, and in August the secondary cell wall carbohydrates were being deposited. Both tissues contained lignin that was more condensed and had more end-groups than the reference lignin. In mid-June, the developing xylem had a ratio of syringyl to guaiacyl units of 0.6, whereas the ratio for the reference wood was 1.3.

In the final study, lignin from the primary cell walls from a hybrid aspen cell suspension culture was investigated. The lignin contained only guaiacyl units which were more condensed than those observed in the reference poplar wood.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 53 p.
Series
TRITA-FPT-Report, ISSN 1652-2443 ; 2006:16
Keyword
Lignin, thioacidolysis, primary wall, middle lamella, Populus balsamifera, Populus tremula L. x P. tremuloides Michx., Picea abies, Picea glauca
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-4036 (URN)
Public defence
2006-06-15, Sal F3, Lindstedtsvägen 26, Stockholm, 14:00
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Note

QC 20100920

Available from: 2006-06-02 Created: 2006-06-02 Last updated: 2016-12-21Bibliographically approved

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