Composition of lignin in outer cell-wall layers
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. Accurate data relating to the lignin monomer composition and the number of β-O-4´ bonds were obtained from pure middle lamella/primary cell wall lignin. Firstly, a 10 000 year old white spruce material, with most of the secondary cell wall missing, was studied. The aged lignin was composed of guaiacyl units only, and was slightly more condensed but otherwise similar to the reference lignin.
Secondly, the developing xylem of a Norway spruce clone was analyzed during a growth season. In spring and early summer, growth is very rapid and the intention was to sample tissues in which the secondary cell-wall layers had not yet lignified, but where the outer layers at least had started to lignify. Microscopy, Klason lignin and carbohydrate analyses showed that the lignin in the developing xylem of samples from mid-June was located exclusively in the middle lamella. The lignin was more condensed, was composed of guaiacyl units only and contained more end-groups than the reference Norway spruce wood.
Thirdly, the cambial tissues of a Balsam poplar clone were surveyed during a growth season. Both the phloem side and the xylem side of the cambial region were examined. The Klason lignin content and carbohydrate monomer distribution showed that in June and August the tissues on the phloem side contained material with mainly middle lamella/primary walls. In June, the xylem side in the cambial region contained mainly middle lamella/primary walls, and in August the secondary cell wall carbohydrates were being deposited. Both tissues contained lignin that was more condensed and had more end-groups than the reference lignin. In mid-June, the developing xylem had a ratio of syringyl to guaiacyl units of 0.6, whereas the ratio for the reference wood was 1.3.
In the final study, lignin from the primary cell walls from a hybrid aspen cell suspension culture was investigated. The lignin contained only guaiacyl units which were more condensed than those observed in the reference poplar wood.
Place, publisher, year, edition, pages
Stockholm: KTH , 2006. , 53 p.
Trita-FPT-Report, ISSN 1652-2443 ; 2006:16
Lignin, thioacidolysis, primary wall, middle lamella, Populus balsamifera, Populus tremula L. x P. tremuloides Michx., Picea abies, Picea glauca
Paper, Pulp and Fiber Technology
IdentifiersURN: urn:nbn:se:kth:diva-4036OAI: oai:DiVA.org:kth-4036DiVA: diva2:10498
2006-06-15, Sal F3, Lindstedtsvägen 26, Stockholm, 14:00
Brunow, Gösta, Professor
QC 201009202006-06-022006-06-022010-09-20Bibliographically approved
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