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Tissue microarray profiling in human heart failure
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2016 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861Article in journal (Refereed) Published
Abstract [en]

Tissue MicroArrays (TMAs) are a versatile tool for high-throughput protein screening, allowing qualitative analysis of a large number of samples on a single slide. We have developed a customizable TMA system that uniquely utilizes cryopreserved human cardiac samples from both heart failure and donor patients to produce formalin-fixed paraffin-embedded sections. Confirmatory upstream or downstream molecular studies can then be performed on the same (biobanked) cryopreserved tissue. In a pilot study, we applied our TMAs to screen for the expression of four-and-a-half LIM-domain 2 (FHL2), a member of the four-and-a-half LIM family. This protein has been implicated in the pathogenesis of heart failure in a variety of animal models. While FHL2 is abundant in the heart, not much is known about its expression in human heart failure. For this purpose, we generated an affinity-purified rabbit polyclonal anti-human FHL2 antibody. Our TMAs allowed high-throughput profiling of FHL2 protein using qualitative and semiquantitative immunohistochemistry that proved complementary to Western blot analysis. We demonstrated a significant relative reduction in FHL2 protein expression across different forms of human heart failure.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2016.
Keyword [en]
Cardiomyopathy, Human heart failure, Lim proteins, Protein arrays, Proteomic profiling, Tissue microarray
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-195458DOI: 10.1002/pmic.201600135ISI: 000383611400003ScopusID: 2-s2.0-84985947161OAI: diva2:1050241

QC 20161128

Available from: 2016-11-28 Created: 2016-11-03 Last updated: 2016-12-01Bibliographically approved

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Pontén, Fredrik
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Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
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