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Tuning microfluidic cell culture conditions for droplet based screening by metabolite profiling
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Novo Nordisk Foundation Center for Biosustainability.ORCID iD: 0000-0002-3722-5970
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Novo Nordisk Foundation Center for Biosustainability.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Novo Nordisk Foundation Center for Biosustainability.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Novo Nordisk Foundation Center for Biosustainability.ORCID iD: 0000-0001-5232-0805
2015 (English)In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2015, 1377-1379 p.Conference paper (Refereed)
Abstract [en]

We investigate the impact of droplet culture conditions on cell metabolic state by determining key metabolite concentrations in S. cerevisiae cultures in different microfluidic droplet culture formats. Control of culture conditions is critical for single cell screening in droplets, as cell metabolic state directly affects production yields in cell factories. Metabolite profiling provides a more nuanced estimate of cell state compared to proliferation studies alone. We show that the choice of droplet incubation format has an impact on cell proliferation and metabolite production. Furthermore, we engineered a new better oxygenated droplet incubation format, with retained droplet stability and size.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2015. 1377-1379 p.
Keyword [en]
Cell culture, Cell metabolism, Droplet microfluidics, Biomolecules, Cell proliferation, Cells, Dimensional stability, Drops, Metabolism, Metabolites, Microfluidics, Culture conditions, Metabolite concentrations, Metabolite production, Metabolite profiling, Microfluidic cell culture, Microfluidic droplets, Cytology
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-194643ScopusID: 2-s2.0-84983371580ISBN: 9780979806483 (print)OAI: oai:DiVA.org:kth-194643DiVA: diva2:1050475
Conference
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, 25 October 2015 through 29 October 2015
Note

QC 20161129

Available from: 2016-11-29 Created: 2016-10-31 Last updated: 2017-02-28Bibliographically approved

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Björk, S. M.Sjöström, S. L.Andersson-Svahn, HeleneJönsson, Håkan N.
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