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Comparative "Golgi" Proteome Study of Lolium multiflorum and Populus trichocarpa
KTH, School of Biotechnology (BIO), Glycoscience.ORCID iD: 0000-0003-1877-4154
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2016 (English)In: PROTEOMES, ISSN 2227-7382, Vol. 4, no 3, 23Article in journal (Refereed) Published
Abstract [en]

The Golgi apparatus (GA) is a crucial organelle in the biosynthesis of non-cellulosic polysaccharides, glycoproteins and proteoglycans that are primarily destined for secretion to the cell surface (plasma membrane, cell wall and apoplast). Only a small proportion of the proteins involved in these processes have been identified in plants, with the majority of their functions still unknown. The availability of a GA proteome would greatly assist plant biochemists, cell and molecular biologists in determining the precise function of the cell wall-related proteins. There has been some progress towards defining the GA proteome in the model plant system Arabidopsis thaliana, yet in commercially important species, such as either the cereals or woody species there has been relatively less progress. In this study, we applied discontinuous sucrose gradient centrifugation to partially enrich GA from suspension cell cultures (SCCs) and combined this with stable isotope labelling (iTRAQ) to determine protein sub-cellular locations. Results from a representative grass species, Italian ryegrass (Lolium multiflorum) and a dicot species, black cottonwood (Populus trichocarpa) are compared. The results confirm that membrane fractionation approaches that provide effective GA-enriched fractions for proteomic analyses in Arabidopsis are much less effective in the species examined here and highlight the complexity of the GA, both within and between species.

Place, publisher, year, edition, pages
MDPI AG , 2016. Vol. 4, no 3, 23
Keyword [en]
Golgi apparatus, sub-cellular fractionation, subcellular proteomics, quantitative proteomics
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-196440DOI: 10.3390/proteomes4030023ISI: 000385569300004OAI: oai:DiVA.org:kth-196440DiVA: diva2:1050534
Note

QC 20161129

Available from: 2016-11-29 Created: 2016-11-14 Last updated: 2016-11-29Bibliographically approved

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CiteExportLink to record
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