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Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0003-0064-0086
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2015 (English)In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2015, p. 1005-1007Conference paper, Published paper (Refereed)
Abstract [en]

In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2015. p. 1005-1007
Keywords [en]
Cell lysis, Microfluidic chip, Mixing, Ultrasound, Vortexing, Extraction, Fluidic devices, Laminar flow, Ultrasonics, Cell disruption, Induced heating, Langevin transducers, Multi-functional, Sample preparation, Microfluidics
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-194658Scopus ID: 2-s2.0-84983260679ISBN: 9780979806483 (print)OAI: oai:DiVA.org:kth-194658DiVA, id: diva2:1050601
Conference
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, 25 October 2015 through 29 October 2015
Note

QC 20161129

Available from: 2016-11-29 Created: 2016-10-31 Last updated: 2016-11-29Bibliographically approved

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