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Characterization of natural killer cell immune surveillance against solid liver tumors
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0001-5178-7593
KTH, School of Engineering Sciences (SCI), Applied Physics.
2015 (English)In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2015, p. 915-917Conference paper, Published paper (Refereed)
Abstract [en]

We demonstrate a method for investigating natural killer (NK) cell aggression against ultrasound-assisted human hepatocellular carcinoma (HCC) HepG2 solid tumors in a multi-well microdevice. We quantify the activity of human primary IL-2 activated NK cells against HepG2 tumors for up to five days and we present the correlation between NK cell numbers versus average tumor volume and final tumor outcome (growth or defeat). We suggest future applications on formation of tumors originated from primary tumors cells and other tumor components as well as primary NK originating from the patient for use in personalized immunotherapy.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2015. p. 915-917
Keywords [en]
HepG2 liver cells, Microchip, Multi-well, Natural killer cells, Solid tumor, Ultrasound, Cells, Cytology, Ultrasonics, Liver cells, Multi wells, Solid tumors, Tumors
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-194661Scopus ID: 2-s2.0-84983261934ISBN: 9780979806483 (print)OAI: oai:DiVA.org:kth-194661DiVA, id: diva2:1050762
Conference
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, 25 October 2015 through 29 October 2015
Note

QC 20161130

Available from: 2016-11-30 Created: 2016-10-31 Last updated: 2016-11-30Bibliographically approved

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Christakou, AthanasiaOhlin, MathiasÖnfelt, BjörnWiklund, Martin
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