Change search
ReferencesLink to record
Permanent link

Direct link
ANLN is a prognostic biomarker independent of Ki-67 and essential for cell cycle progression in primary breast cancer
Show others and affiliations
2016 (English)In: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 16, 904Article in journal (Refereed) Published
Abstract [en]

Background: Anillin (ANLN), an actin-binding protein required for cytokinesis, has recently been presented as part of a prognostic marker panel in breast cancer. The objective of the current study was to further explore the prognostic and functional value of ANLN as a single biomarker in breast cancer. Methods: Immunohistochemical assessment of ANLN protein expression was performed in two well characterized breast cancer cohorts (n = 484) with long-term clinical follow-up data and the results were further validated at the mRNA level in a publicly available transcriptomics dataset. The functional relevance of ANLN was investigated in two breast cancer cell lines using RNA interference. Results: High nuclear fraction of ANLN in breast tumor cells was significantly associated with large tumor size, high histological grade, high proliferation rate, hormone receptor negative tumors and poor prognosis in both examined cohorts. Multivariable analysis showed that the association between ANLN and survival was significantly independent of age in cohort I and significantly independent of proliferation, as assessed by Ki-67 expression in tumor cells, age, tumor size, ER and PR status, HER2 status and nodal status in cohort II. Analysis of ANLN mRNA expression confirmed that high expression of ANLN was significantly correlated to poor overall survival in breast cancer patients. Consistent with the role of ANLN during cytokinesis, transient knock-down of ANLN protein expression in breast cancer cell lines resulted in an increase of senescent cells and an accumulation of cells in the G2/M phase of the cell cycle with altered cell morphology including large, poly-nucleated cells. Moreover, ANLN siRNA knockdown also resulted in decreased expression of cyclins D1, A2 and B1. Conclusions: ANLN expression in breast cancer cells plays an important role during cell division and a high fraction of nuclear ANLN expression in tumor cells is correlated to poor prognosis in breast cancer patients, independent of Ki-67, tumor size, hormone receptor status, HER2 status, nodal status and age.

Place, publisher, year, edition, pages
BioMed Central, 2016. Vol. 16, 904
Keyword [en]
ANLN, Prognostic biomarker, Breast cancer, Proliferation, Antibody-based proteomics
National Category
Medical and Health Sciences
URN: urn:nbn:se:kth:diva-197768DOI: 10.1186/s12885-016-2923-8ISI: 000388038200007PubMedID: 27863473ScopusID: 2-s2.0-84998694458OAI: diva2:1060706

QC 20161229

Available from: 2016-12-29 Created: 2016-12-08 Last updated: 2016-12-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Uhlén, Mathias
By organisation
Science for Life Laboratory, SciLifeLab
In the same journal
BMC Cancer
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 2 hits
ReferencesLink to record
Permanent link

Direct link