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Harnessing Yeast Peroxisomes for Biosynthesis of Fatty-Acid-Derived Biofuels and Chemicals with Relieved Side-Pathway Competition
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2016 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 138, no 47, p. 15368-15377Article in journal (Refereed) Published
Abstract [en]

Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing pathways and/or insufficient precursor supply. Compartmentalization in cellular organelles can isolate synthetic pathways from competing pathways, and provide a compact and suitable environment for biosynthesis. Peroxisomes are cellular organelles where fatty acids are degraded, a process that is inhibited under typical fermentation conditions making them an interesting workhouse for production of fatty-acid-derived molecules. Here, we show that targeting synthetic pathways to peroxisomes can increase the production of fatty-acid-derived fatty alcohols, alkanes and olefins up to 700%. In addition, we demonstrate that biosynthesis of these chemicals in the peroxisomes results in significantly decreased accumulation of byproducts formed by competing enzymes. We further demonstrate that production can be enhanced up to 3-fold by increasing the peroxisome population. The strategies described here could be used for production of other chemicals, especially acyl-CoA-derived molecules.

Place, publisher, year, edition, pages
American Chemical Society , 2016. Vol. 138, no 47, p. 15368-15377
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Chemical Sciences
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URN: urn:nbn:se:kth:diva-199532DOI: 10.1021/jacs.6b07394ISI: 000389160500014Scopus ID: 2-s2.0-85000786739OAI: oai:DiVA.org:kth-199532DiVA, id: diva2:1065743
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QC 20170116

Available from: 2017-01-16 Created: 2017-01-09 Last updated: 2017-11-29Bibliographically approved

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Nielsen, Jens

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