Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lubrication synergy: Mixture of hyaluronan and dipalmitoylphosphatidylcholine (DPPC) vesicles
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
Show others and affiliations
2017 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 488, p. 225-233Article in journal (Refereed) Published
Abstract [en]

Phospholipids and hyaluronan have been implied to fulfil important roles in synovial joint lubrication. Since both components are present in synovial fluid, self-assembly structures formed by them should also be present. We demonstrate by small angle X-ray scattering that hyaluronan associates with the outer shell of dipalmitoylphophatidylcholine (DPPC) vesicles in bulk solution. Further, we follow adsorption to silica from mixed hyaluronan/DPPC vesicle solution by Quartz Crystal Microbalance with Dissipation measurements. Atomic Force Microscope imaging visualises the adsorbed layer structure consisting of non-homogeneous phospholipid bilayer with hyaluronan/DPPC aggregates on top. The presence of these aggregates generates a long-range repulsive surface force as two such surfaces are brought together. However, the aggregates are easily deformed, partly rearranged into multilayer structures and partly removed from between the surfaces under high loads. These layers offer very low friction coefficient (<0.01), high load bearing capacity (approximate to 23 MPa), and self-healing ability. Surface bound DPPC/hyaluronan aggregates provide a means for accumulation of lubricating DPPC molecules on sliding surfaces.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 488, p. 225-233
Keywords [en]
Dipalmitoylphosphatidylcholine (DPPC), Hyaluronan, Adsorption, Self-assembly, QCM-D, AFM imaging, Surface forces, Friction, Lubrication, Small-angle X-ray scattering
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-199458DOI: 10.1016/j.jcis.2016.10.091ISI: 000389785500025Scopus ID: 2-s2.0-84994491737OAI: oai:DiVA.org:kth-199458DiVA, id: diva2:1067941
Note

QC 20170123

Available from: 2017-01-23 Created: 2017-01-09 Last updated: 2017-11-29Bibliographically approved
In thesis
1. Synergies in Biolubrication
Open this publication in new window or tab >>Synergies in Biolubrication
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The objective of this thesis was to advance understanding in the field of biolubrication, finding inspiration from the human synovial joints. This was addressed by investigating the association of key biolubricants and the resulting lubrication performance. Techniques employed during the course of this work were Atomic force microscopy (AFM), Quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray reflectivity (XRR).

Key synovial fluid and cartilage components like dipalmitoylphosphatidylcholine (DPPC), hyaluronan (HA), lubricin, and cartilage oligomeric matrix protein (COMP) have been used in the investigations. Focus was towards two lubrication couples; DPPC-hyaluronan and COMP-lubricin. DPPC-hyaluronan mixtures were probed on hydrophilic silica surfaces and COMP-lubricin association structures were explored on weakly hydrophobic poly (methyl methacrylate) (PMMA) surfaces.

Investigations of the COMP-lubricin pair revealed that individually these components are unable to reach desired lubrication. However in combination, COMP facilitates firm attachment of lubricin to the PMMA surface in a favourable confirmation that imparts low friction coefficient.

DPPC and hyaluronan combined impart lubrication advantage over lone DPPC bilayers. Hyaluronan provides a reservoir of DPPC on the surface and consequently self-healing ability.

Other factors like temperature, presence of calcium ions, molecular weight of hyaluronan, and pressure were also explored. DPPC bilayers at higher temperature had higher load bearing capacity. Association between DPPC Langmuir layers and hyaluronan was enhanced in the presence of calcium ions, and lower molecular weight hyaluronan had a stronger tendency to bind to DPPC. At high pressures, DPPC-hyaluronan layers were more stable compared to lone DPPC bilayers.

Place, publisher, year, edition, pages
Sweden: KTH Royal Institute of Technology, 2017. p. 66
Series
TRITA-CHE-Report, ISSN 1654-1081
Keywords
Biolubrication, Synergies, Adsorption, Surface Force, Friction, Load Bearing Capacity, Self Healing, Phospholipids, DPPC, Hyaluronan, COMP, Lubricin, QCM-D, AFM, XRR.
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-201219 (URN)978-91-7729-268-5 (ISBN)
Public defence
2017-03-17, Kollegiesalen, KTH, Brinellvägen 8, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20170210

Available from: 2017-02-10 Created: 2017-02-09 Last updated: 2017-02-13Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Raj, AkankshaAn, JunxueFielden, MatthewClaesson, Per M.

Search in DiVA

By author/editor
Raj, AkankshaWang, MinLiu, XiaoyanAn, JunxueFielden, MatthewClaesson, Per M.Dedinaite, Andra
By organisation
Surface and Corrosion ScienceApplied Physics
In the same journal
Journal of Colloid and Interface Science
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 49 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf