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Glass Capillary based cavity resonator for particle trapping study and bacteria up-concentration
KTH. mafaridi@kth.se. (Clinical Microfluidics)ORCID iD: 0000-0003-1176-0905
KTH. (Clinical Microfluidics)ORCID iD: 0000-0003-0064-0086
(Clinical Microfluidics)ORCID iD: 0000-0001-5199-0663
(Centre of Molecular Chemistry, Polymer Chemistry & Biomaterials Research Group Ghent University)
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(English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781Article in journal (Refereed) Submitted
Abstract [en]

We have performed particle aggregation characterization on the basis of their material and suspending

medium in a capillary-based cavity resonator used for acoustophoresis. We have investigated the experimental

aggregation time of 5μm polystyrene and silica particles, size of aggregate, number of trapped particles and upconcentration

factor in water, 0.01M phosphate buffered saline (PBS) and 0.005M PBS at 1.97MHz and with

actuation voltages between 4, 8 and 12Vpp. We have found that there is little difference between using water and

PBS as suspension medium, approximately 5-10% longer trapping times with PBS compared with water.

However we get approx. 5.5 times faster trapping time for silica than for polystyrene. It is also observed and

calculated that silica particle aggregates have 3.4 times larger area than the polystyrene aggregates using the same

starting particle concentrations, revealing similar amount of difference in trapped number of particles. The upconcentration

factor for silica is also about 3.2 times higher than that of polystyrene due to larger aggregate area

of silica particles. Based on theoretical predictions and experimental characterization of the particle aggregation

pattern, we note that the particle-particle interaction force contribution to the total acoustic radiation force is more

pronounced for silica than for polystyrene. Finally as a proof of principle for biomedical sample preparation

application we demonstrate the capillary-based silica particles mediated bacteria acoustophoretic upconcentration.

This setup could potentially be utilized not only for sample preparation application but also for

bead based affinity immunoassays.

National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-200301OAI: oai:DiVA.org:kth-200301DiVA, id: diva2:1068016
Funder
EU, FP7, Seventh Framework Programme, 115153-2Swedish Research Council, 2011-5230Stockholm County Council, HMT-20140722
Note

QCR 20170124

Available from: 2017-01-24 Created: 2017-01-24 Last updated: 2017-11-29Bibliographically approved
In thesis
1. Bioparticle Manipulation using Acoustophoresis and Inertial Microfluidics
Open this publication in new window or tab >>Bioparticle Manipulation using Acoustophoresis and Inertial Microfluidics
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Despite the many promising advances made in microfluidics, sample preparation remains the single largest challenge and bottleneck in the field of miniaturised diagnostics. This thesis is focused on the development of sample preparation methods using active and passive particle manipulation techniques for point of care diagnostic applications. The active technique is based on acoustophoresis (acoustic manipulation) while the passive method is based on inertial microfluidics (hydrodynamic manipulation). In paper I, acoustic capillary-based cavity resonator was used to study aggregation of silica and polystyrene particles. We found that silica particles show faster aggregation time (5.5 times) and larger average area of aggregates (3.4 times) in comparison to polystyrene particles under the same actuation procedure. The silica particles were then used for acoustic based bacteria up-concentration. In paper II, a microfluidic-based microbubbles activated acoustic cell sorting technique was developed for affinity based cell separation. As a proof of principle, separation of cancer cell line in a suspension with better than 75% efficiency is demonstrated. For the passive sample preparation, inertial and elasto-inertial microfluidic approach that uses geometry-induced hydrodynamic forces for continuous size-based sorting of particles in a flow-through fashion were studied and applied for blood processing (paper III-V). In paper III, a simple ushaped curved channel was used for inertial microfluidics based enrichment of white blood cells from diluted whole blood. A filtration efficiency of 78% was achieved at a flow rate of 2.2 ml/min. In paper IV, elasto-inertial microfluidics where viscoelastic flow enables size-based migration of cells into a non- Newtonian solution, was used to continuously separate bacteria from unprocessed whole blood for sepsis diagnostics. Bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample. Finally, in paper V, the inertial and elasto-inertial techniques were combined with a detection platform to demonstrate an integrated miniaturized flow cytometer. The all-optical-fiber technology based system allows for simultaneous measurements of fluorescent and scattering data at 2500 particles/s. The use of inertial and acoustic techniques for sample preparation and development of an integrated detection platform may allow for further development and realization of point of care testing (POCT) systems.

Place, publisher, year, edition, pages
Stockholm, Sweden: Kungliga Tekniska högskolan, 2017. p. 68
Series
TRITA-BIO-Report, ISSN 1654-2312 ; 2017:4
National Category
Medical Biotechnology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-200304 (URN)978-91-7729-264-7 (ISBN)
Public defence
2017-02-16, Gard-Aulan, Nobels vägen 18, Solna, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20170124

Available from: 2017-01-24 Created: 2017-01-24 Last updated: 2017-01-24Bibliographically approved

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