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FZD10-Gα13 signalling axis points to a role of FZD10 in CNS angiogenesis
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2017 (English)In: Cellular Signalling, ISSN 0898-6568, E-ISSN 1873-3913, Vol. 32, p. 93-103Article in journal (Refereed) Published
Abstract [en]

Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD10 remains the most enigmatic. FZD10 shows homology to FZD4 and FZD9 and was previously implicated in both β-catenin-dependent and –independent signalling. In normal tissue, FZD10 levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD10 as a receptor interacting with and signalling through the heterotrimeric G protein Gα13 but not Gα12 Gαi1 GαoA Gαs, or Gαq. Stimulation with the FZD agonist WNT induced the dissociation of the Gα13 protein from FZD10, and led to global Gα12/13–dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD10 mediates Gα12/13 activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD10 in embryonic CNS endothelial cells at E11.5–E14.5. Given the well-known importance of Gα13 signalling for the development of the vascular system, the selective expression of FZD10 in brain vascular endothelial cells points at a potential role of FZD10-Gα13 signalling in CNS angiogenesis.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 32, p. 93-103
Keywords [en]
Angiogenesis, Endothelial cell, FZD, GNA13, GPCR, WNT, YAP/TAZ
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-201118DOI: 10.1016/j.cellsig.2017.01.023ISI: 000395953400010Scopus ID: 2-s2.0-85010799974OAI: oai:DiVA.org:kth-201118DiVA, id: diva2:1072624
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research Council, 2011-2435, 2015-02899Swedish Cancer Society, CAN 2014/659Knut and Alice Wallenberg Foundation, KAW2008.0149]
Note

QC 20170208

Available from: 2017-02-08 Created: 2017-02-08 Last updated: 2017-04-28Bibliographically approved

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