Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Combined microfluidic-optical DNA analysis with single-base-pair sizing capability
KTH, School of Information and Communication Technology (ICT), Materials- and Nano Physics. University of Twente, Netherlands.
2016 (English)In: Biomedical Optics Express, ISSN 2156-7085, E-ISSN 2156-7085, Vol. 7, no 12, p. 5201-5207, article id 275075Article in journal (Refereed) Published
Abstract [en]

DNA sequencing by microchip capillary electrophoresis (CE) enables cheap, highspeed analysis of low reagent volumes. One of its potential applications is the identification of genomic deletions or insertions associated with genetic illnesses. Detecting single base-pair insertions or deletions from DNA fragments in the diagnostically relevant size range of 150−1000 base-pairs requires a variance of σ2 < 10−3. In a microfluidic chip post-processed by femtosecond-laser writing of an optical waveguide we CE-separated 12 blue-labeled and 23 red-labeled DNA fragments in size. Each set was excited by either of two lasers powermodulated at different frequencies, their fluorescence detected by a photomultiplier, and blue and red signals distinguished by Fourier analysis. We tested different calibration strategies. Choice of the fluorescent label as well as the applied fit function strongly influence the obtained variance, whereas fluctuations between two consecutive experiments are less detrimental in a laboratory environment. We demonstrate a variance of σ2 ≈4 × 10−4, lower than required for the detection of single base-pair insertion or deletion in an optofluidic chip.

Place, publisher, year, edition, pages
Optical Society of America, 2016. Vol. 7, no 12, p. 5201-5207, article id 275075
Keywords [en]
Integrated optics devices, Medical optics instrumentation
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-201493DOI: 10.1364/BOE.7.005201ISI: 000390490700029Scopus ID: 2-s2.0-85004168852OAI: oai:DiVA.org:kth-201493DiVA, id: diva2:1073452
Note

QC 20170210

Available from: 2017-02-10 Created: 2017-02-10 Last updated: 2017-11-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Pollnau, Markus
By organisation
Materials- and Nano Physics
In the same journal
Biomedical Optics Express
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 7 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf