Catalog of gene expression in adult neural stem cells and their in vivo microenvironment
2006 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 312, no 10, 1798-1812 p.Article in journal (Refereed) Published
Stem cells generally reside in a stem cell micro environment, where cues for self-renewal and differentiation are present. However, the genetic program underlying stem cell proliferation and multipotency is poorly understood. Transcriptome analysis of stem cells and their in vivo microenvironment is one way of uncovering the unique sternness properties and provides a framework for the elucidation of stem cell function. Here, we characterize the gene expression profile of the in vivo neural stem cell microenvironment in the lateral ventricle wall of adult mouse brain and of in vitro proliferating neural stem cells. We have also analyzed an Lhx2-expressing hematopoietic-stem-cell-like cell line in order to define the transcriptome of a well-characterized and pure cell population with stem cell characteristics. We report the generation, assembly and annotation of 50,792 high-quality 5'-end expressed sequence tag sequences. We further describe a shared expression of 1065 transcripts by all three stem cell libraries and a large overlap with previously published gene expression signatures for neural stem/progenitor cells and other multipotent stem cells. The sequences and cDNA clones obtained within this framework provide a comprehensive resource for the analysis of genes in adult stem cells that can accelerate future stem cell research.
Place, publisher, year, edition, pages
2006. Vol. 312, no 10, 1798-1812 p.
Digital signature, EST, Gene expression, Lateral ventricle wall, Microenvironment, Neural stem cell, Neurosphere, Transcriptome
IdentifiersURN: urn:nbn:se:kth:diva-6166DOI: 10.1016/j.yexcr.2006.02.012ISI: 000238179100010ScopusID: 2-s2.0-33646714932OAI: oai:DiVA.org:kth-6166DiVA: diva2:10798
QC 201009022006-09-222006-09-222010-09-02Bibliographically approved